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Figure 4 | Cell Communication and Signaling

Figure 4

From: Analysis of detergent-free lipid rafts isolated from CD4+ T cell line: interaction with antigen presenting cells promotes coalescing of lipid rafts

Figure 4

Analysis of detergent-free lipid rafts by Raft ELISA. Lipid rafts were isolated from T cell - APC co-cultures. YH16.33 cells were co-cultured with A20 cells in the absence (A) or presence (B) of 1 mg/ml chicken ovalbumin (specific antigen) for 18-24 hours and lipid rafts were isolated using the detergent-free density gradient method. Each density gradient was analyzed by Raft ELISA by capturing with anti-Thy-1 and detecting with biotinylated anti-Ly-6A.2 followed by avidin-HRP. A representative analysis of at least 3 independent experiments is shown.

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Cell Communication and Signaling

ISSN: 1478-811X