Figure 1

Treatment of cells with LiCl and alsterpaullone reduces cell proliferation. (A.I-A.VI) Wild type (wt) and p53-deficient mouse embryonic fibroblasts (MEF) and HCT116 cells as well as U2OS and SaOs-2 cells were plated in 96-well plates at a density of 1 × 10³ cells per well. 24 hours after plating, LiCl and alsterpaullone (Alster) were added at the indicated concentrations. 72 hours after drug addition, the relative number of viable cells was determined by MTT-assay. Mean values and standard deviations of three independent experiments were calculated and plotted. The relative number of cells in the absence of drug was set to 100% (D: DMSO control). (B.I-B.VI) Wild type (wt) and p53-deficient mouse embryonic fibroblasts (MEF) and HCT116 cells as well as U2OS and SaOs2 cells were plated in 24-well plates at a density of 5 × 10³ cells per well. 24 hours after plating, LiCl and alsterpaullone (Alster) were added at a concentration approximating LD₅₀ as determined by the MTT assay (A.I-A.VI). U2OS cells and MEFs received a final concentration of 17.5 mM LiCl or 1.5 μM alsterpaullone, SaOs-2 cells were treated with 25 mM LiCl or with 1.5 μM alsterpaullone and HCT116 cells received 12.5 mM LiCl and 0.6 μM alsterpaullone. Cells were counted at the indicated days. Mean values and standard deviations of three independent experiments were calculated and plotted.