Figure 5

The effect of hypoxia on glucose metabolism-associated gene expression which may also represent HIF-1α target genes. Gene expression was detected by Real-Time RT-PCR using primers for glucose-6-phosphate dehydrogenase (G6PD), glucose transporter-1 (GLUT-1), lactate dehydrogenase A (LDHA), pyruvate dehydrogenase kinase-1 (PDK-1) with hypoxanthine phosphoribosyltransferase-1 expression as an internal control. The data represent the expression levels in the UC-derived MSC populations at 1.5% O₂ (black bars), 2.5% O₂ (dark grey bars) and 5% O₂ (light grey bars) and compared to the steady state expression levels of normoxic control cultures at 21% O₂ (dashed line). Data represent the mean ± SD of three independent experiments.