Figure 4
From: Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells
The effect of hypoxia (1.5% O 2 ) on apoptosis was tested by the measurement of caspase 3/7 activity (A) and cell damage or necrosis was tested by the measurement of LDH activity in the cell culture supernatant (B) of the 4 different donors of UC-derived stem cells. All measurements were normalized to 10,000 cells whereby the values of all measurements were calculated compared to the normoxic control conditions (21% O2) at 100%.. Data represent the mean ± SD for three independent measurements of each donor.