Gamma-secretase inhibition combined with platinum compounds enhances cell death in a large subset of colorectal cancer cells

Table 1 Effects of γ-secretase inhibitors and cisplatin on a panel of CRC cell lines

CRC line	Val1744-NICD	DAPT 10 μM	L-685,458 5 μM	DBZ 300 nM	Cisplatin cell death (20 μM,48 h)	CP+DBZ combi effect CD (48 h)
C80	+	-	-	-	+	+
Caco-2	+	-	-	-	+	+
CCK-81	+	-	CD	-	(+)	(+)
CC07	+	-	-	-	+	+
HCA-7	+ (N)	-	-	-	+	++(24 h)
HCA-46	+ (N)	-	-	-	(+)	+
HDC-9	-	-	-	-	+	-
HDC-57	-	-	-	-	-	-
HDC-73	-	-	-	-	+	-
LoVo	+ (N)	-	-	-	+	(+)
LS123	+	-	-	-	+	-
VACO 4A	+	-	CD	-	-	-

CD = cell death visible by light microscopy
CP = cisplatin
CP+DBZ = combination treatment with 10 μM CP and 300 nM DBZ
DAPT = N- [N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester
DBZ = (S, S)-2- [2-(3,5-Difluorophenyl)acetylamino]-N-(5-methyl-6-oxo-6,7-dihydro-5H-dibenzo [b, d]azepin-7-yl)propionamide
(N) = Val1744-NICD detected in nuclear fraction after cell fractionation
NICD = Notch1 intracellular domain
- (minus symbol) = no visible effect on proliferation, cell death or cell morphology

ISSN: 1478-811X