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Figure 3 | Cell Communication and Signaling

Figure 3

From: An active form of Vav1 induces migration of mammary epithelial cells by stimulating secretion of an epidermal growth factor receptor ligand

Figure 3

Different Vav1Y3F domains are required to increase levels of Rac1-GTP and phosphorylated Pak and ERK1/2. (A) After overnight starvation, MCF-10A cells expressing GFP or Vav1Y3F were left unstimulated or stimulated with EGF. PBD pulldown assays were performed as described in the Materials and Methods. Levels of Rac1-GTP and total Rac1 are shown as indicated. Data are representative of 4 independent experiments. (B) MCF-10A cells expressing GFP, Vav1Y3F, and Vav1Y3F/DH* were treated as in panel A and PBD assays were performed. In addition, whole cell lysate (WCL) samples were blotted for Vav1Y3FGFP, phosphorylated Pak, total Pak, phosphorylated ERK1/2, and actin as indicated. Data are representative of 2 independent experiments. (C) Levels of Rac1-GTP, total Rac1, Vav1Y3FGFP, and phosphorylated and total Pak, ERK1/2, and Akt in lysates from MCF-10A cells expressing GFP, Vav1Y3F, Vav1Y3F/SH2*, or Vav1Y3F/C-SH3* were determined as in panel B. Data are representative of 3 independent experiments.

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Cell Communication and Signaling

ISSN: 1478-811X