Figure 2

Effects of MAP kinase manipulation on b2/640 type X collagen promoter activity, in chick cephalic sternum chondrocytes. A and B: Mutant kinases; 24 hrs after seeding, cells were transfected with luciferase vectors and 0.5–1 μg mutant kinase DNA. After 5 hrs medium was changed (A) or medium changed and BMP-2 added (B). C and D: Inhibitor treatments; 5 hours after transfection with luciferase vectors, medium was changed and kinase inhibitor or vehicle (DMSO) was added as indicated. In D 30 ng/ml BMP-2 was added one hr after medium change. Data obtained using at least 2 independent isolates of chondrocytes, assayed in triplicate. Values are mean ± SEM of luciferase ratios of type X collagen promoter activity to control vector, normalized to BMP-2 treated controls. *:p < 0.05 that luciferase ratio differs from non BMP-2 treated group, +: p < 0.05 that luciferase ratio differs from group with no MAP kinase manipulation.