Figure 3

Absorption ELISA to check the binding of anti-K19M-AF to CCN3 in liquid phase. A fixed dilution of anti-K19M-AF (1/250 in 1% BSA) was mixed with a) serial dilutions of K19M peptide -- --- --- -- b) serial dilutions of H295R supernatant, positive for CCN-3 --- --- ---; c). G59 supernatant, negative for CCN-3 ---x----x---; d). 1% BSA in PBS pH 7.4 --- --- --- -- and applied to wells coated with K19M peptide. Each point repsent the average of 4 determinations.