Figure 1

Western blot analysis. Representative gels illustrating the expression of CCN3 proteins in H295R, G59/540, and G59 cell lines. Conditioned medium and cellular lysates were electrophoresed under denaturing conditions and immunoblotted with anti-K19M IgG antibody. A. Starts: 1). supernatant collected form H295R cell line; 2). G59/540 transfected cells supernatant; 3). supernatant from G59 CCN3 negative cells; 4). lysate from H295R cells; 5). lysate from G59/540 cells; 6). lysate from G59 cells showing two bands at 48 kDa and 30 kDa.