Figure 4
Effect of oxysterols in intercellular communication through gap-junctions. LEC were treated with 20 μg/ml cholesterol (B), 7-keto (C) or 25-OH (D) for 3 hours. The cells were assayed for intercellular communication by Lucifer yellow dye transfer, following scrape loading. The intercellular communication was evaluated as the average distance travelled by the dye Lucifer yellow along the monolayer and is represented as an histogram. The values are the average of three individual experiments ± SD. * – indicates statistically significant differences from controls (p < 0.05).