Figure 6

PGE ₂ -dependent effect on β-catenin expression in NE-4C cells. Western blot analysis was used to determine two forms of active β-catenin: (A) non-phospho-(active) β-catenin (Ser33/37/Thr41) and (B) phospho-β-catenin (Ser552) (92 kDa). Addition of PGE₂ to NE-4C cells did not yield a significant difference in levels of either active form of β-catenin compared to control. The error bars represent + SEM and values were considered significantly different from control at *p < 0.05, **p < 0.01. Average measurements represent protein from three independent experiments (N = 3). β-Actin was used to indicate equal loading.