Figure 3

Transfection of HeLa cells by fluorescently labeled self-delivering sdRNA via exosomes. The recipient cells were treated for 24 h with exosomes loaded with the sdRNA and washed several times through a 100-kDa filter (Exo + sdRNA + WF). The sample prepared in identical way but without adding of exosomes was used (sdRNA + WF) as a control. The cells were also transfected with sdRNA by standard procedure (sdRNA). A, flow cytometry analysis of HeLa cells transfected by sdRNA. B-D, representative images of HeLa cells 24 h after transfection.