Figure 2

In vitro delivery of fluorescently labeled siRNA via chemically loaded exosomes. The recipient cells were treated for 24 h with exosomes loaded with Lipofectamine-formulated siRNA and washed several times through a 100-kDa filter (Exo + siRNA + LP + WF). The sample prepared in identical way but without adding of exosomes was used (siRNA + LP + WF) as a control. The cells were also transfected with siRNA by standard procedure (siRNA + LP). A, flow cytometry analysis of HeLa cells transfected by siRNA. B-D, representative images of HeLa cells 24 h after transfection.