Figure 2
From: The proteases HtrA2/Omi and UCH-L1 regulate TNF-induced necroptosis
Identification of HtrA2/Omi as a candidate serine protease involved in necroptosis. L929Ts cells were stimulated with 100 ng/ml TNF for 5 h in combination with 20 μM zVAD-fmk, 2 μg/ml CHX (to enhance necroptosis) and 50 μM FAM-FFCK. Subsequently, lysates from the cells were separated by 2D gel electrophoresis and analyzed for protein spots labeled by FAM-FFCK (left panel, green), or for the total of all separated protein spots by staining with Flamingo Pink (right panel, red). The protein spot subsequently identified by mass spectrometry as HtrA2/Omi is indicated by arrows.