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Figure 8 | Cell Communication and Signaling

Figure 8

From: Direct transdifferentiation of spermatogonial stem cells to morphological, phenotypic and functional hepatocyte-like cells via the ERK1/2 and Smad2/3 signaling pathways and the inactivation of cyclin A, cyclin B and cyclin E

Figure 8

Expression of Smad2-, and Stat3- phosphorylation and Oct-4 in SSCs, hepatic stem-like cells, small hepatocytes, and mature hepatocyte-like cells. (A) Immunocytochemistry revealed expression of Expression of phosph-Smad2 in SSCs (Panel i), hepatic stem-like cells (Panel ii), small hepatocytes (Panel iii), and mature hepatocyte-like cells (Panel iv). (B) Expression of phosph-Stat3 in SSCs (Panel i), hepatic stem-like cells (Panel ii), small hepatocytes (Panel iii), and mature hepatocyte-like cells (Panel iv). Scale bars in A-B = 50 μm. (C) Western blots showed the expression of phosph-Smad2 in SSCs (lane 1), hepatic stem-like cells (lane 2), and mature hepatocyte-like cells (lane 3). (D) RT-PCR revealed Oct-4 expression in SSCs (lane 1), hepatic stem-like cells (lane 2), and mature hepatocyte-like cells (lane 3).

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Cell Communication and Signaling

ISSN: 1478-811X