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Figure 3 | Cell Communication and Signaling

Figure 3

From: Direct transdifferentiation of spermatogonial stem cells to morphological, phenotypic and functional hepatocyte-like cells via the ERK1/2 and Smad2/3 signaling pathways and the inactivation of cyclin A, cyclin B and cyclin E

Figure 3

The expression of OV6, CYP1A2, ALB, CK8, and CK19 of hepatic stem-like cells derived from transdifferentiation of SSCs. (A-D) Immunocytochemistry showed protein expression of OV6 (A), CYP1A2 (B), ALB (C), as well as co-expression of CK8 and CK19 (D) in transdifferentiated cells derived from C18-4 cells. Scale bars in A, B, C, and D = 20 μm. (E) Flow cytometry showed expression of CK8 in the transdifferentiated cells derived from C18-4 cells in the conditioned medium without PD98059 (left panel) or with PD98059 (right panel). (F-G) Immunocytochemistry showed protein expression of ALB (F) as well as co-expression of CK8 and CK19 (G) in transdifferentiated cells derived from mouse primary SSCs. Scale bars in F and G = 20 μm.

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Cell Communication and Signaling

ISSN: 1478-811X