Skip to main content
Figure 2 | Cell Communication and Signaling

Figure 2

From: Direct transdifferentiation of spermatogonial stem cells to morphological, phenotypic and functional hepatocyte-like cells via the ERK1/2 and Smad2/3 signaling pathways and the inactivation of cyclin A, cyclin B and cyclin E

Figure 2

Transcriptional characteristics of transdifferentiation of SSCs into hepatic stem-like cells and hepatocyte-like cells. (A) RT-PCR revealed mRNA expression of Ck8, Ck18, Ck7, and Ck19 in hepatic stem-like cells derived from SSCs. (B) RT-PCR showed the transcripts of Ck8, Ck18, Cyp1a2, Cyp7a1, Hnf3b, Hnf4a, Alb, Ta t, and Ttr in SSCs (lane 1), SSC induction for 7 days (lane 2), SSC induction for 10 days (lane 3), small hepatocytes (lane 4), and mature hepatocyte-like cells (lane 5) derived from SSCs. The expression of these genes in liver tissues of adult mice (lane 6) was used as positive controls. (C) The transcription of Ck8, Ck18, and Ck7 in mature hepatocyte-like cells derived from SSCs. Gapdh was used as loading controls of total RNA. (D) The transcription of Ck18, Ck7 and Ck19 in the cells derived from primary mouse SSCs. Gapdh was used as loading controls of total RNA. (E) RT-PCR showed the transcripts of Ck8, Ck18, Cyp1a2, Cyp7a1, Hnf3b, Hnf4a, Alb, Ta t, and Ttr in mature hepatocyte-like cells derived from primary SSCs. Gapdh was used as loading controls of total RNA.

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X