Figure 7From: Cucurbitacin covalent bonding to cysteine thiols: the filamentous-actin severing protein Cofilin1 as an exemplary targetSubcellular localization of Cofilin1. Increasing concentrations of cucurbitacin E (CuE) from 3 nM to 300 nM were incubated with MCF7 cells for 4Â h, followed by fixation and staining for F-actin structures and Cofilin1 subcellular localization. Despite evident effects on F-actin and cell morphology at 30 nM and 300 nM cucurbitacin E, Cofilin1 distribution was not observably changed.Back to article page