Table 6 Summary of phenotypes observed in the sec7 - strain
Phenotype | sec7 - |
|---|---|
Cell size | wt |
Nuclei number | wt |
Growth in axenic medium (measure of macropinocytosis) | wt |
Growth on E. coli in shaken suspension | wt |
Growth on a lawn of Klebsiella | reduced |
Phagocytosis of yeast cells | uptake reduced to 60% of AX2 level |
Development | wt |
Cell substrate adhesion | wt |
Cell cell adhesion | wt |
Phototaxis | directionality impaired, slug trail length reduced |
Localization of F-actin and actin associated proteins (actin, CAP) | wt |
Integrity of ER membrane system (PDI staining) | wt |
Secretion of α-mannosidase | secretion occurs normally, however enzyme levels are reduced (~76% of AX2 level) |
Secretion of phosphodiesterase | secretion appears to be impaired, enzyme levels are reduced (~50% of AX2 level) |
F-actin assembly after cAMP stimulation | wt |
Cell motility | speed reduced |
Persistence during migration | lower |