The cytohesin paralog Sec7 of Dictyostelium discoideum is required for phagocytosis and cell motility

Table 2 Ability of sec7 ^- cells to secrete enzymes during early development: secretion of α-Mannosidase

Strain	% secreted enzyme after		% total enzyme activity after 6 hours
	2 hours	6 hours
AX2	60	75	100
sec7 ^-	64	77.4	75.80

Cells were starved in Soerensen phosphate buffer, pH 6.0, and at the indicated time points the α-mannosidase activity was determined. α-mannosidase secretion in percent and total α-mannosidase activity in percent are shown. The α-mannosidase activity was determined in the medium and in the cell pellet at the indicated time points after the beginning of starvation. Values are from one representative experiment.

ISSN: 1478-811X