Figure 5

Analysis of sec7^-cells. ( A ) For immunofluorescence analysis AX2 and sec7^- cells were fixed with methanol and stained with mAb act1 to visualize the actin cytoskeleton, with mAb 223-451-1 to stain for actin associated CAP, and mAb 221-135-1 to visualize the ER marker PDI. Bar, 10 μm. ( B ) Growth on a lawn of Klebsiella. The growth of AX2 and mutant cells was followed over several days. The curves represent the measurements from more than one independent experiments in which six plates per strain were analyzed each. ( C ) Phototactic movement is altered in sec7^-. In phototaxis experiments AX2 slugs migrate in a highly directed fashion towards the light source. At the end of the experiment they have reached the edge of the plate. sec7^- slugs migrate in a wider angle towards the light and have shorter trails (left panels). The behavior of sec7^- GFP-Sec7 slugs was improved. The position of the light source is indicated. The angle of deviation during slug phototaxis is significantly altered (bar graph on the right). The data are collected from between 6 and 10 experiments per strain. Each experiment included ~10 slugs. Unpaired t test was used for evaluation. P value is less than 0.0001 for sec7^-, and for sec7^- GFP-Sec7 it is 0.0016.