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Figure 3 | Cell Communication and Signaling

Figure 3

From: The cytohesin paralog Sec7 of Dictyostelium discoideum is required for phagocytosis and cell motility

Figure 3

Subcellular localization of GFP-Sec7. To determine the localization of Sec7 we expressed a GFP-tagged Sec7 in Sec7 deficient cells. ( A ) GFP-Sec7 in methanol fixed cells. The cells were stained for annexin 7 with mAb 185-338-1 to reveal the plasma membrane. Annexin 7 is present in the cytosol, in the nucleus and at the plasma membrane. Bar, 5 μm. ( B ) Live cell analysis of GFP-Sec7 expressing cells. Representative images of a series are shown. Bar, 10 μm. ( C ) Live cell analysis of a GFP-Sec7 expressing cells during phagosome formation. Yeast particles were used to study phagocytosis. The time is given in seconds. Bar, 10 μm.

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Cell Communication and Signaling

ISSN: 1478-811X