Figure 2
From: ARTD10 substrate identification on protein microarrays: regulation of GSK3β by mono-ADP-ribosylation
Validation of the ProtoArray® results on diverse recombinant proteins. ADP-ribosylation assays with [32P]-NAD+ as cofactor, analyzed by SDS-PAGE, coomassie blue (CB) staining and autoradiography with A. TAP-ARTD10-G888W or TAP-ARTD10 and BSA or PDGF-B B. TAP-ARTD10 and IKKε or GSK3 β, or the negative control without ARTD10 C. TAP-ARTD10 and ACVR1(171-499), or the negative control without ARTD10 D. TAP-ARTD10 and P-TEFb, or the negative control without ARTD10 E. His-ARTD8 and GST-ING5, or the negative control without His-ARTD8.