Table 1 A meta-map of Gab2 phosphorylation

S2

X

Y48

X

X

X

Y49

X

X

S132

X

S133

X

X

X

S140

X

X

X

S141

X

X

X

S146

X

S148

X

X

X

S149

X

X

X

S159

Western (Figure 1A)

X

X

Negative regulatory site; AKT substrate [21]

S164

X

X

T171

X

X

Y194

X

S210

X

X

X

14-3-3 binding and negative regulatory site [19, 23]

S218

X

X

X

X

S223

X

X

X

T225

X

Y249

X

CrkL/PLCγ [13]

X

S250

X

X

S264

X

X

X

X and phosphorylated [57]

T265

X

X

Y266

X

X

CrkL/PLCγ [13]

X

T278

X

X

S281

X

X

X

T283

X

S285

X

T287

X

X

X

Y293

X

X

X

CrkL [13]

X

T294

X

T319

X

X

Y324

X

CrkL/PLCγ [13]

X

T329

X

X

T331

X

X

T343

X

S368

X

X

T385

X

X

X

T391

X

X

X

14-3-3 binding and negative regulatory site [19, 23]

X and phosphorylated [57]

S404

X

X

X

S405

X

X

X

X

T408

X

Y409

X

X

X

Y411

X

S422

X

X and phosphorylated [57]

S425

X

S427

X

S443

X

S448

X

Y452

X

X

p85 recruitment site; Resilient against IM and DST in K562 cells [9]

X

S472

X

X

S474

X

X

Y476

X

X

S480

X

X

X

X

S488

X

X

X

Y491

X

X

S506

X

S543

X

X

X

ERK dependent phosphorylation of the S543 equivalent critical for PH-domain mediated membrane recruitment of Gab1 [56]. ERK consensus motif (PxxSP) conserved in Gab2

X

S550

X

Y563

X

Y584

X

p85 [13]

X

S611

X

Y614

X

Shp2 [13]

X

S622

X

X

X

X

S623

X

X

X

S625

X

X

X

S631

X

X

T632

X

S633

X

S634

X

T636

X

X

X

S637

X

X

Y643

X

X

Shp2 [13]

X