Figure 5

Inhibitor-dependent effects on Bcr-Abl and Gab2 downstream effectors. (A) K562tet cells stably transfected with the inducible Gab2^wt expression vector or ‘empty’ control vector [9] were treated with 1 μg/ml dox for 24 h and then exposed to either 1 μM imatinib or dasatinib or to the same volume of DMSO (vehicle control) for 4 h. Total cellular lysates were analyzed by Western blotting using the indicated antibodies. (B) The murine proB cell line Ba/F3 was transduced with pBabe-Hygro-Bcr-Abl and subsequently with either pWZL-Bsr-Gab2-HA, or pWZL-Bsr empty control vector [9]. Cells were exposed to either 1 μM imatinib or dasatinib or to the same volume of DMSO (vehicle control) for 1 h. Total cellular lysates were analyzed by Western blotting using the indicated antibodies. Please note that the Western blot B from this Figure and the one from Figure 1B belong to the same experiments and that the Gab2 and β-Actin panels are shown on both Figures.