Figure 5
From: Cytosolic Ca2+ shifts as early markers of cytotoxicity
Assessment of As 2 O 3 , gossypol, H 2 O 2 and staurosporine-induced toxicity in murine fibroblasts. (A) Upper panel: Cells were treated with increasing concentrations of As2O3 as indicated and afterwards cell viability was analysed with Alamar Blue (mean±SD; n≥4). Lower panel: Fluo-4 analysis of 45 μM and 50 μM As2O3 treated cells (mean±SD; *p<0.0001; n≥4; t test) (B) Upper panel: Cells were treated with increasing concentrations of gossypol as indicated and afterwards cell viability was analysed with Alamar Blue (mean±SD; n≥4). Lower panel: Fluo-4 analysis of 75 μM and 100 μM gossypol treated cells (mean±SD; *p<0.05; n=4; t test) (C) Upper panel: Alamar Blue assay in presence of H2O2 as indicated (mean±SD; n≥3). Lower panel: Fluo-4 analysis of 0.5 mM and 5 mM H2O2 treated cells (mean±SD; *p<0.01; n≥3; t test) (D) Upper panel: Alamar Blue assay in presence of staurosporine as indicated (mean±SD; n≥3). Lower panel: Fluo-4 analysis of 500 nM and 4000 nM staurosporine treated cells (mean±SD; n. s. not significant; n≥3; t test).