Figure 2

APAP-induced remote lung injury was not reduced following extracellular ATP cleavage or P2 receptors blockage. (A) Lung histology from control mice showing normal morphological findings, while APAP-treated mice presented marked lung inflammation, similarly to apyrase treated group (25 U/mice). Representative images from five different mice/group. (B) Leukocyte numbers in bronchial-alveolar lavage (BAL) from APAP treat mice showing that liver injury triggered remote lung inflammation, which was not prevented by dampening ATP sensing. (C) Macrophages were the most frequent cell type found in BAL. * - P < 0.05 in comparison to control group, ANOVA followed by Bonferroni post-test. Data are mean ± s.e.m. N = 5/group. Data are mean ± SEM. Scale: 100 μm.