Figure 6From: Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichmentMeasurement of neutrophil numbers and Cxcl1 expression in Control and TgS100a8a9hepmice treated with doxycycline. Control (Co) and TgS100a8a9hep (Tg) mice were treated with (Co +dox, Tg +dox) or without (Co -dox, Tg -dox) doxycycline (10 μg/ml) containing drinking water. (A) Relative expression of Cxcl1 was determined by qRT-PCR on liver cDNA. Mean +SD, students t-test, *p≤0.05, **p≤0.01, n.s. not significant. (B) Peripheral blood cells were stained with specific antibodies for Cd11b (APC), Gr1 (PE) and Ly-6G (FITC) followed by flow cytometry analysis. Box-plot shows the relative fold increase of Gr1+/Ly-6G+ population from all Cd11b+ cells in peripheral blood; Median, 25% and 75% quartile (light grey boxes), students t-test, *p≤0.05. (C) IHC staining with Gr1-specific antibodies was performed on liver sections and representative images (n=4 animals per group) are shown with red staining for positively stained cells and counterstaining with hematoxilin. Bars represent 200 μm.Back to article page