Figure 5
Chemokine levels in liver, primary hepatocytes, and serum of Control and TgS100a8a9hepmice. (A) Relative expression of Cxcl1, Cxcl2 and Csf1 was determined by qRT-PCR analysis on liver cDNA from Control (n=6) and TgS100a8a9hep (n=9) mice and is depicted as mean +SD. (B) Primary hepatocytes were isolated from Control and TgS100a8a9hep mice and cultivated in vitro for 24 hours. Relative expression of Cxcl1, Cxcl2, Csf1 was measured by qRT-PCR on cDNA from primary hepatocytes. Two biological replicates for each group were measured in triplicates and means are depicted +SD, students t-test, *p≤0.05. (C) Protein levels of Cxcl1, Cxcl2, and Csf3 in serum samples from Control and TgS100a8a9hep (n≥4) mice were measured by ELISA. Mean +SD, students t-test, *p≤0.05.