Figure 3From: Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichmentIncreased amount of neutrophils in peripheral tissue and blood samples. (A) Relative expression of endogenous S100a8 transcripts was determined by qRT-PCR using cDNA from different tissues of Control (n=3) and TgS100a8a9hep (n=4) mice. Mean +SD, students t-test, *p≤0.05, **p≤0.01, n.s. not significant. (B) Leukocyte subpopulations in the peripheral blood from Control and TgS100a8a9hep mice (n=4) were quantified and depicted as relative cell number. Box-plot shows median, 25 % and 75 % quartile (light and dark grey box), students t-test, **p≤0.01. (C-D) Peripheral blood from Control and TgS100a8a9hep mice (n=7) was subjected to tri-color staining using antibodies against Cd11b (APC), Gr1 (PE), and Ly-6G (FITC). Graphs show the total percentage of Gr1+/Ly-6G+ cells (C) and Gr1+/Ly-6G- cells (D) from all Cd11b+ cells in peripheral blood. Box-plot shows median, 25 % and 75 % quartile (light grey box), students t-test, *p≤0.05, n.s. not significant.Back to article page