Figure 3

MTT assay. MTT assay for the in vitro culture of dissociated mesenchymal and epithelial cells to determine the optimal Fibroblast growth factor-9 (FGF-9) concentration. (A) MTT data for mesenchymal cells (n = 4, p < 0.5): FGF-9 concentrations of 25 and 40 ng/ml were both suitable for cell culture in the first 7 days. # : 40 ng/ml FGF-9 outperformed 25 ng/ml after 14 d of culture, (B) MTT for epithelial cells (n = 4, p < 0.5): 40 ng/ml is optimal for epithelial cell culture within the first 183 h (> 7 days). Thus, we chose 40 ng/ml FGF-9 for further experimentations.