Phosphatases in toll-like receptors signaling: the unfairly-forgotten

Abstract Over the past 2 decades, pattern recognition receptors (PRRs) have been shown to be on the front line of many illnesses such as autoimmune, inflammatory, and neurodegenerative diseases as well as allergies and cancer. Among PRRs, toll-like receptors (TLRs) are the most studied family. Dissecting TLRs signaling turned out to be advantageous to elaborate efficient treatments to cure autoimmune and chronic inflammatory disorders. However, a broad understanding of TLR effectors is required to propose a better range of cures. In addition to kinases and E3 ubiquitin ligases, phosphatases emerge as important regulators of TLRs signaling mediated by NF-κB, type I interferons (IFN I) and Mitogen-Activated Protein Kinases signaling pathways. Here, we review recent knowledge on TLRs signaling modulation by different classes and subclasses of phosphatases. Thus, it becomes more and more evident that phosphatases could represent novel therapeutic targets to control pathogenic TLRs signaling. Graphic abstract Video Abstract


Background
Toll-Like Receptors (TLRs) belong to the Pattern Recognition Receptors (PRRs) superfamily that recognizes Microbe-Associated Molecular Patterns (MAMPs)-not necessarily found on pathogens-, PAMPs (Pathogen) and DAMPs (Damage) [1]. There are 13 identified TLRs in mammals playing an instrumental role in the regulation of the innate immune system [2]. These receptors are highly conserved proteins also found in the phylum Cnidaria, including jellyfishes [3]. As TLRs evolved before the adaptive immune system, they constitute an indispensable first line of defense [4]. Accordingly, TLRs expression is not restricted to immune cell lineages. TLR3 and TLR5 mRNAs are detected ubiquitously within the human body, while other TLRs are mostly expressed in epithelial tissues (except TLR10, more restricted) [5]. Thus, because they constitute the first line of defense against microbes (see below), TLRs are involved in pathogenesis of various disorders. Several Single Nucleotide Polymorphisms (SNPs) of TLRs, their co-receptors and some adaptors are indeed associated with several diseases including infections, atherosclerosis, asthma, chronic cardiomyopathy and colorectal cancer [6,7].
TLRs are divided into two groups: those localized at the plasma membrane and those localized at the endosomal membrane [8]. Plasma membrane-localized TLRs recognize conserved motifs on extracellular microorganisms such as bacteria, fungi, protozoan and helminth parasites [9]. Yet, plasma membrane TLRs are also able to bind DAMPs. The most glaring example is TLR4 that is wellknown to recognize Lipopolysaccharide (LPS), a component of Gram-negative bacteria, and 28 other ligands including DAMPs [10]. This wide range of recognition is allowed by combinations between TLRs homodimers or heterodimers with co-receptors and/or accessory molecules [11]. The other plasma membrane TLRs include TLR1, TLR2 and TLR6 which bind various lipopeptides from Gram-positive bacteria and TLR5 which binds flagellin from flagellated bacteria [9].
Notably, half of the TLRs exhibit both plasma membrane and endosomal localizations. For instance, in intestinal epithelial cells exposed to bacterial DNA, TLR9 moves from the endosomal compartment to the cytoplasmic membrane [14]. In macrophages, membraneassociated TLR4 reaches the endosomal compartment through endocytosis [15]. A similar process has been described for TLR2 which is endocytosed after its heterodimerization with TLR1 or TLR6 in myeloid cells [16].
Binding of ligands to TLRs triggers the activation of several intracellular signaling pathways involving protein phosphorylation, dephosphorylation and ubiquitination. While the roles of specific protein kinases in this process have been well described [17,18], much less is known about the implications of phosphatases, particularly tyrosine phosphatases. In this review, we summarize how specific phosphatases regulate TLRs signaling and function.

Rapid tyrosine phosphorylation following TLRs engagement
TLRs are type I membrane glycoproteins with an ectodomain consisting of Leucine-Rich Repeats (LRRs), responsible for the molecular recognition of ligands [19]. TLRs contain also a cytoplasmic domain called Toll/Interleukin-1 Receptor (TIR) domain, which recruits several adapter proteins including Myeloid Differentiation primary response 88 (MyD88), Toll/Interleukin-1 Receptor domain-containing Adapter Protein (TIRAP) or TIR-domain-containing adapter-inducing Interferon-β (TRIF). Upon ligand binding, TLRs undergo conformational changes inducing homodimerization or heterodimerization which unveil TIR domains which can now recruit and bind different downstream signaling effectors regulating the host inflammatory response [20].
Notably, the TIR domain of TLR2, 3, 4, 5, 8 and 9 is rapidly phosphorylated on tyrosine upon stimulation with their respective ligands. This phosphorylation is required for the recruitment of adapter proteins and subsequent activation of the downstream signaling cascades [17,21,22]. Several tyrosine kinases interact with TLRs, including Src, Bruton's Tyrosine Kinase (BTK), Lyn and Syk. The Focal Adhesion Kinase (FAK) is also rapidly activated upon ligand binding to TLR. Indeed, FAK phosphorylation is observed in macrophages isolated from mice deficient for MyD88, one of the first adapter molecule recruited following TLR4 activation (see below), suggesting that FAK activation occurs early after TLR activation [23]. FAK is indeed required for TLR4 downstream signaling since cytokine induction in response to LPS is totally abrogated in Fak −/− cells. However, FAK does not possess TIR domains for direct interaction with MyD88 or TLR4 [24]. Instead, activated FAK phosphorylates BTK which interacts with the TIR domains of TLR4 (but also of TLR6, 8, and 9) [25]. BTK phosphorylation by FAK opens its conformation [26] allowing its phosphorylation and activation by Src kinases (Fig. 1). Once activated, BTK phosphorylates different adapter molecules such as the bridging adapter TIRAP, facilitating Myd88 recruitment to TLR4 [27]. Interestingly, BTK can also phosphorylate TLR3 and this is required for downstream signaling [28]. However, BTK does not phosphorylate TRIF, but rather facilitates TRIF interaction with effectors Since BTK expression is restricted to myeloid cells, ETK, a member of the TEC family of tyrosine kinases, is suspected to play similar functions in other cell types such as epithelial cells.
Aside from these observations, tyrosine phosphorylation of TLR3 and TLR4 also increased in response to EGF (Epidermal Growth Factor), and inhibition of EGFR (EGF Receptor) kinase activity impaired activation of their downstream signaling. In fact, the Src family kinases mediated this crosstalk between EGFR and TLRs, triggering the recruitment of adapters and other effector proteins [31,32].
Altogether, these observations strongly suggest that a coordinated interplay should exist between tyrosine kinases and phosphatases in order to tightly regulate the activation status of TLRs intracellular signaling and cellular function. Below, we summarize the main signaling pathways activated by TLRs and we discuss how these pathways are modulated by various tyrosine and serine/ threonine phosphatases.
Likewise, TLR3 signaling is initiated from endosomal membranes where it activates TRAF6 and TRAF3. Then, TRIF signals through TRAF3, TBK1, and IKK-ε to initiate IRF-mediated transcription. TRAF3 therefore acts as a critical component to trigger TRIF-dependent signaling pathways (Fig. 3). This is exemplified by the phenotype of Traf3-deficient myeloid and dendritic cells in which IFN production is impaired upon TLR4, TLR7 and TLR9 stimulations [51,52]. Interestingly, analysis of gene expression profiles in dendritic cells carrying a loss of function mutation in Trif reveals that 47% of LPSresponsive genes are TRIF-dependent [53].

The human protein phosphatome
The human protein phosphatome comprises a set of genes encoding phosphatases that remove phosphate groups from proteins. Protein phosphatases were first Activated FAK phosphorylates BTK, opening its conformation and allowing its phosphorylation and activation by Src kinases [29,30]. BTK can then phosphorylate adaptor proteins such as TIRAP classified according to their catalytic domain annotation [54]. Today, phosphatase families are subdivided into classes, according to their preferred substrates [55]. A simplified classification is presented in Table 1.

Classical protein tyrosine phosphatases (PTPs) Protein tyrosine phosphatase 1B (PTP1B)
PTP1B is a cytoplasmic protein tyrosine phosphatase expressed in many tissues [71,72] and which targets a Fig. 2 Regulation of TLR/MyD88-dependent pathways by phosphatases. TLRs localized at the plasma membrane use the bridging adapter TIRAP to recruit MyD88 and activate the NF-κB and MAPK pathways. MyD88 then recruits IRAK4 which phosphorylates IRAK1 which activates TRAF6. TRAF6 induces K63-linked polyubiquitination of TRAF6 itself serving as a platform leading to TAK1 autophosphorylation and activation. Activated TAK1 phosphorylates IKK-α and IKK-β which induce IκBα degradation, allowing NF-κB nuclear translocation and induction of proinflammatory genes transcription. TAK1 also phosphorylates the MAPK Kinases MKK3, MKK4, MKK6 and MKK7, resulting in activation and nuclear translocation of MAP Kinases which phosphorylate several transcription factors inducing proinflammatory mediator genes transcription wide range of substrates [73][74][75]. Notably, Ptp1b gene knockdown in mouse RAW264.7 macrophages results in increased NF-κB and IRFs activation in response to TLR3, TLR4 and TLR9 stimulation [76]. Similarly, macrophages derived from Ptp1b −/− mice exhibit accelerated IκBα degradation following TLR4 activation by LPS [77]. Elevated concentrations of inflammatory cytokines and IFN I were also found in the lungs of Ptp1b −/− mice following intranasal administration of Oligo-Deoxy-Nucleotide 1826 (ODN 1826, a TLR9 agonist) [78]. Thus, these studies suggest that PTP1B restrains TLR4 and TLR9-induced inflammatory responses. Conversely, PTP1B expression levels were significantly increased in the brain 24 h after LPS injection in mice. In addition, when overexpressed in microglial cells, PTP1B increased LPS-induced TNF-α (Tumor Necrosis Factor-α), iNOS Fig. 3 Regulation of TLR/TRIF-dependent pathways by phosphatases. TRIF is recruited directly to TLR3 but indirectly, via TRAM adaptor, to TLR4, following stimulation. TRIF adaptor then recruits TRAF3 which induces K63-linked ubiquitination of TBK1, its autophosphorylation and then, activation. Activated TBK1 then phosphorylates the transcription factors IRF3 and IRF7, leading to homodimerization or heterodimerization, translocation into the nucleus and target genes induction, including IFNI. TLR7, TLR8 and TLR9 activate the TRIF-dependent pathways via the MyD88 adaptor (inducible Nitric Oxide Synthase) and IL-6 (Interleukin 6) expression as well as NO production. It has been demonstrated that PTP1B exerts such pro-inflammatory function by dephosphorylating a negative regulatory site (tyrosine 527) on Src, hence activating its kinase activity and downstream NF-κB signaling [79,80]. These studies suggest cell type-specific roles for PTP1B in TLRs signaling.

Protein tyrosine phosphatase non-receptor type 22 (PTPN22)
PTPN22 is expressed in lymphoid tissues where it promotes type I IFNs production following TLR3 and TLR4 ligation [83]. Mechanistically, it has been shown that PTPN22 directly interacts with TRAF3, enhancing its K63-linked ubiquitination and consequently IFNs induction. In a model of IL-1β-dependent inflammatory arthritis, Ptpn22 −/− mice develop severe arthritis despite Poly I:C treatment, which usually attenuates disease symptoms [84]. Notably, a single nucleotide polymorphism in PTPN22 gene, encoding a tryptophan at amino acid 620, has been described as a susceptibility locus for autoimmune diseases and infections [85]. Accordingly, patients with autoimmune Systemic Lupus Erythematosus (SLE) expressed the PTPN22 R620W variant and this was associated with impaired TLR7 and TLR8-dependent IFN-α induction [86]. These data argue for a protective role of PTPN22 against autoimmune diseases via the regulation of TRAF3-dependent signaling.

Src homology region 2 domain-containing phosphatase-1 (SHP-1)
SHP-1 belongs to the SH2-domain-containing family of non-membrane protein tyrosine phosphatases. It has been broadly studied in immune lineages as its expression is abundant in hematopoietic cells [87]. Several studies have reported that SHP-1 differentially modulates NF-κB and IRF3 activation upon TLR3 and TLR4 stimulation [88]. Indeed, in splenocytes, dendritic cells and peritoneal macrophages, SHP-1 inhibits NF-κBdependent gene induction while it promotes IFN-β production. In fact, SHP-1 directly interacts with IRAK1 which in turn inhibits IRF3 and IRF7 activation through its kinase activity [88,89]. Surprisingly, this regulatory function of SHP-1 is independent from its phosphatase activity since it is also observed in macrophages expressing the catalytically inactive SHP-1 mutant (C453S, Table 1, section "PTPs"). This interaction between SHP-1 and IRAK1 occurs through a ITIM-like motif found in the kinase domain of IRAK1 [90].
Notably, SHP-1 has been associated with autoinflammatory diseases and infections. Macrophages from autoimmune Multiple Sclerosis (MS) patients exhibit deficient SHP-1 gene expression in comparison to normal subjects [91]. In line with these observations, SHP-1 knockdown in normal macrophages increases LPS-mediated NF-κB responses, up to levels observed in macrophages from MS patients [91]. Aside from these observations, treatment of human macrophages with the specific SHP-1 inhibitor Sodium Stibogluconate (SS) [92] reduces LPS-induced production of IL-27 which then inhibits HIV (Human Immunodeficiency Virus) infection in CD4 T cells [93]. These results suggest a pivotal role for SHP-1 in antiviral immunity.
Regarding the MAPK pathways, it has been reported that conditional Shp-2 deletion in murine podocytes attenuates JNK and p38 MAPK activation in response    [119,120] to LPS [99]. Similar observations were reported in LPSstimulated bronchial epithelial cells [100]. However, the molecular mechanisms involved in such regulation remain to be clarified. One could speculate that SHP-2 modulates TRAF6 ubiquitination which in turn modulates TAK1 activity. Additionally, some data suggest that SHP-2 might be involved early following TLR engagement. Indeed, conditional expression of an active SHP-2 mutant in murine endothelial cells blocks LPS-induced barrier disruption and this correlates with increased FAK phosphorylation [101].

Protein tyrosine phosphatase-PEST (PTP-PEST)
PTP-PEST is a protein tyrosine phosphatase containing a PEST motif (enrichment in P: Proline, E: Glutamate, S: Serine and T: Threonine) [102] which is associated with proteins with short half-lives [103]. Interestingly, enhanced PTP-PEST expression is observed in macrophages after long term stimulation with LPS, Poly I:C or ODN [104]. This increased expression is associated with decreased induction of proinflammatory cytokines and secretion of IFN-β. Such regulation depends on phosphatase activity of PTP-PEST, since overexpression of a catalytically inactive PTP-PEST mutant abrogates NF-κB and IRFs signaling inhibition [104]. Additional data indicate that PTP-PEST directly interacts with IKK-β and dephosphorylates two tyrosine residues ( Table 2) [104]. Much more studies are needed to exactly understand how PTP-PEST modulates NF-κB and IRFs signaling following TLRs ligation.

DUal Specificity Phosphatase 1 (DUSP1)
DUSP1 is a nuclear MKP targeting the stress-activated MAPKs p38 and JNK, and which is rapidly up-regulated in response to mitogenic and/or stress stimuli [121]. Interestingly, Dusp1 KO (Knockout) mice exhibit increased sensitivity to endotoxic shock induced by LPS [122]. Indeed, prolonged JNK and p38 MAPK activation as well as increased TNF-α and IL-6 expression are observed in Dusp1 −/− cells [122]. Aside from these observations, macrophages pre-treatment with a pharmacological inhibitor of DUSP1, namely triptolide, overactivates JNK and p38 pathways following LPS [106,107] or the TLR2 agonist peptidoglycan [108] stimulations. Since MAPK activation is necessary for the maximal production of cytokines [123], DUSP1 may act as a pivotal regulator of the innate immune response. In this regard, long-term stimulation of macrophages with peptidoglycan or LPS markedly increases DUSP1 mRNA and protein expression [124].

Protein phosphatase 2A (PP2A)
The ubiquitously expressed serine/threonine phosphatase PP2A accounts for a large fraction of phosphatase activity in eukaryotic cells [130]. Du et al. have shown that PP2A negatively regulates IRF3 activation in macrophages and myeloid cells [117]. In vitro assays demonstrate that PP2A directly dephosphorylates IRF3 (Table 2). In mice, the myeloid-specific knockout of Ppp2a results in higher mortality in response to LPS challenge and bacterial infection [131]. Notably, increased phosphorylation of MAPKs and NF-κB (IKK-α/β, p65) signaling effectors as well as enhanced secretion of pro-inflammatory cytokines were observed in BMDMs (Bone Marrow-Derived Macrophages) from Ppp2a −/− mice [132]. Aside from these observations, it has been shown that PP2A dephosphorylates c-Jun, hence inhibiting its proteasomal degradation in response to LPS [115] (Table 2). Recently, the catalytic PP2A α-subunit was recognized as a novel protective factor for LPS-induced ARDS (Acute Respiratory Distress Syndrome). Indeed, specific ablation of the catalytic subunit of PP2A (Ppp2ca) in alveolar macrophages enhances NF-κB and MAPKs activation and aggravates cytokine secretion in response to LPS [133]. Conversely, adoptive transfer of alveolar macrophages with activated PP2A attenuates lung inflammation. Taken together, these results indicate that PP2A tightly regulates the inflammatory responses induced by TLRs, at least TLR3 and TLR4, by limiting the activation of MAPKs and NF-κB pathways.

Protein phosphatase 4 (PP4)
Protein Phosphatase 4 (PP4) is a PP2A-like phosphatase [134] regulating many cellular processes including DNA damage responses or the cell cycle [135,136]. Notably, PP4 physically interacts with the E3 ubiquitin ligase TRAF6 [137]. Silencing PP4 in RAW264.7 macrophages increases NF-κB luciferase activity following TLR4 stimulation [137]. PP4 may also negatively modulate the NF-κB pathway downstream of TLR4, in part by inhibiting TRAF6 ubiquitination in response to LPS. Interestingly, PP4 expression is significantly upregulated in macrophages after long-time treatment with LPS [137]. Recently, PP4 has been shown to suppress IFN I production upon TLR3 and TLR4 stimulations in a phosphatasedependent manner [118]. Indeed, siRNA-mediated reduction of the expression of the PP4 catalytic subunit in mouse peritoneal macrophages in vivo resulted in increased IFN I expression after viral infection. In addition, it was shown that PP4 direct interaction with the kinase TBK1 leads to dephosphorylation of serine 172 (Table 2) [118] which is located in the TBK1 activation loop, necessary for kinase activity and downstream IRF3 phosphorylation [138]. Thus, PP4 also acts as a potent negative regulator of TLR-mediated antiviral immunity.

Wild-type p53-induced phosphatase 1 (WIP1)
WIP1 is a member of the serine/threonine protein phosphatase PP2C family. WIP1 dephosphorylates the NF-κB subunit p65 on serine 536 within its transactivation domain [139] in response to LPS [119] (Table 2). Mice lacking Wip1 exhibit increased p65 phosphorylation and expression of target genes following LPS injection. Recently, a negative feedback loop between WIP1 and NF-κB in LPS-induced astrocytes was discovered [120]. Primary astrocytes LPS-stimulated increases Wip1 transcription and WIP1 protein nuclear colocalization with p65. Conversely, Wip1 silencing in primary astrocytes results in reduced p65 phosphorylation and cytokine transcription following TLR4 activation [120]. Collectively, these results suggest that WIP1 may provide a potent therapeutic target for neuroinflammation, and more generally for chronic inflammatory disorders (Table 3).

Intestinal alkaline phosphatase (IAP)
IAP is expressed in the brush border of enterocytes where it plays a key role in gut defense [140]. Interestingly, this phosphatase can also be secreted in both the intestinal lumen and bloodstream [141,142]. In contrast to other phosphatases, IAP does not dephosphorylate proteins. Instead, its reported substrates include bacterial products such as LPS, flagellin and CpG. Hence, IAP plays a crucial role in the regulation of gut microbiota function [143]. For example, by removing phosphates present on the lipid A moiety (which allows LPS to bind TLR4), IAP reduces LPS toxicity [144]. Accordingly, ectopic IAP expression in intestinal epithelial cells and colon cancer cells markedly reduces LPS-induced NF-κB responses [145]. Likewise, oral IAP administration impairs colitis induction in response to Dextran Sulfate Sodium (DSS) in wild-type mice but not in Tlr4 −/− mice [146]. Therefore, IAP protects against colitis by reducing TLR4 pathways activation. Interestingly, the gut expresses three isozymes for IAP: Akp3, Akp5 and Akp6. Akp3 (coding for IAP3) KO mice exhibit normal basal intestinal MyD88-inflammatory cytokines levels and similar susceptibilities to Gram-Yersinia pseudotuberculosis infection when compared to control mice. However, adult Akp3 −/− mice are immune tolerant to low intestinal dose of LPS. Such endotoxin tolerance, acquired post-weaning, may result from higher TLR4 stimulation during development [141]. This suggests IAP's LPS detoxifying activity is involved in immune education [147].

Clinical relevance
As TLRs and some adaptors are involved in many human disorders such as atherosclerosis [6], phosphatases regulating TLR signaling may also be implicated. For example, it has been suggested that DUSP1 is athero-protective, as DUSP1 induction is necessary for the anti-inflammatory effects of shear stress in endothelial cells [148]. In addition, Khadir et al. identified circulating DUSP1 as a potential biomarker for chronic inflammation in patients with cardiovascular diseases associated with atherosclerosis [149]. Cheng et al. have shown that Geniposide, an emerging immunomodulator [150], is anti-inflammatory in part by upregulating Dusp1 expression in response to LPS [151]. Geniposide reduces atherosclerotic inflammatory injuries in ApoE −/− mice which are prone to atherosclerosis [152]. To date, no treatment against rheumatoid arthritis achieved to target its triggering events. However, it has recently been proposed to screen for PTPN22 gene signatures, in order to predict patient responses to autoimmune rheumatoid arthritis targeted therapies [153]. PTPN22 has also been recommended as a novel rheumatoid arthritis therapeutic target [154], as well as PP2A for neuroinflammatory disorders [155]. Finally, daily administration of Alkaline Phosphatase has been shown to significantly improve patients ulcerative colitis scores, with clinical effects observed within 21 days [156]. Thus, phosphatases may be directly involved in the regulation of human disorders such as atherosclerosis and are currently recognized as pharmacological targets.

Coronavirus disease-2019 (COVID-19)
Recent data suggest a role of TLR signaling and phosphatases in patient responses to Coronavirus infection. For example, Mizutani et al. observed that p38 phosphorylation was significantly elevated in SARS-Coronavirus (SARS-CoV)-infected cells [157]. Inhibition of p38 phosphorylation almost abolished IL-6 and IL-8 induction. These first results are especially promising for targeting phosphatases to treat SARS. In 2011, an evasion strategy for the IBV (Infectious Bronchitis Virus) coronavirus (IBV) was revealed [158]. Indeed, IBV induces DUSP1 expression, correlating with reduced IL-6 and IL-8 secretion. IL-6 and IL-8 releases are part of the cytokine storm, responsible for the Severe Acute Respiratory Syndrome (SARS). COVID-19 is the most recent coronavirus-mediated acute respiratory illness generating severe symptoms. TLR4 has been identified as a potential receptor for the outer protein spike of SARS-CoV-2 [159]. It has been reported that COVID-19 patients upregulate TLR4-mediated signaling, which  [119] exacerbates SARS [160]. The more critically ill patients present highly increased S100 calcium-binding protein A9 (S100A9) blood levels, a TLR4-recognized DAMP. In addition, neutralizing autoantibodies against IFN I were detected in patients with life-threatening COVID-19 [161]. Rare putative loss-of-function variants of X-chromosomal TLR7 are associated with impaired type I IFN in young men with severe COVID-19 [162].
Knowing that both TLR4 and TLR7 trigger NF-κB/ MAPK activation and antiviral IFN I, and that these pathways may be involved in SARS-related infections, il would be relevant to consider a role for phosphatases in TLRs signaling during COVID-19 infections.

Conclusion
This review highlights that phosphatases are key players in TLRs signaling. Interestingly, downregulation of most phosphatases markedly decreased or completely abolished LPS tolerance, highlighting the importance of phosphatases in endotoxin tolerization. In this regard, increased expression and activity of PP2A, PTPN22, PTP1B and MKP1 are observed in LPS-tolerized monocytes and macrophages [124]. One could speculate that phosphatases represent attractive targets to control TLRs-induced inflammatory responses. However, unlike kinases, phosphatases are challenging targets against which to develop specific inhibitors or inducers [163]. Their catalytic sites are permissive, rather shallow and greatly polar, making them hard to target. Recent progress in allosteric or oligomerization inhibitors design reveals new chemical tools that will set future therapies [164]. That being said, there are still numerous outstanding questions at the molecular level that remain to be addressed before considering phosphatases as good therapeutic targets to control TLRs functions. For instance, which PTPs dephosphorylate tyrosine phosphorylated TLRs? How exactly TLRs control the activity of regulatory phosphatases to ensure timely proinflammatory responses? Is that modulation cell type-dependent? Further studies are warranted to dissect the kinase-phosphatase network regulating TLRs signaling pathways.