Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells

Background Syndecans regulate cell migration thus having key roles in scarring and wound healing processes. Our previous results have shown that Thy-1/CD90 can engage both αvβ3 integrin and Syndecan-4 expressed on the surface of astrocytes to induce cell migration. Despite a well-described role of Syndecan-4 during cell movement, information is scarce regarding specific Syndecan-4 partners involved in Thy-1/CD90-stimulated cell migration. Methods Mass spectrometry (MS) analysis of complexes precipitated with the Syndecan-4 cytoplasmic tail peptide was used to identify potential Syndecan-4-binding partners. The interactions found by MS were validated by immunoprecipitation and proximity ligation assays. The conducted research employed an array of genetic, biochemical and pharmacological approaches, including: PAR-3, Syndecan-4 and Tiam1 silencing, active Rac1 GEFs affinity precipitation, and video microscopy. Results We identified PAR-3 as a Syndecan-4-binding protein. Its interaction depended on the carboxy-terminal EFYA sequence present on Syndecan-4. In astrocytes where PAR-3 expression was reduced, Thy-1-induced cell migration and focal adhesion disassembly was impaired. This effect was associated with a sustained Focal Adhesion Kinase activation in the siRNA-PAR-3 treated cells. Our data also show that Thy-1/CD90 activates Tiam1, a PAR-3 effector. Additionally, we found that after Syndecan-4 silencing, Tiam1 activation was decreased and it was no longer recruited to the membrane. Syndecan-4/PAR-3 interaction and the alteration in focal adhesion dynamics were validated in mouse embryonic fibroblast (MEF) cells, thereby identifying this novel Syndecan-4/PAR-3 signaling complex as a general mechanism for mesenchymal cell migration involved in Thy-1/CD90 stimulation. Conclusions The newly identified Syndecan-4/PAR-3 signaling complex participates in Thy-1/CD90-induced focal adhesion disassembly in mesenchymal cells. The mechanism involves focal adhesion kinase dephosphorylation and Tiam1 activation downstream of Syndecan-4/PAR-3 signaling complex formation. Additionally, PAR-3 is defined here as a novel adhesome-associated component with an essential role in focal adhesion disassembly during polarized cell migration. These novel findings uncover signaling mechanisms regulating cell migration, thereby opening up new avenues for future research on Syndecan-4/PAR-3 signaling in processes such as wound healing and scarring. Graphical abstract


Plain English summary
Cell movement is crucial during wound healing and scaring processes. To move, cells need to coordinately protrude and form points of adhesion (or focal adhesions) with the surrounding matrix (substrate) at one end of their body (head) and disassemble these focal adhesions on the opposite side (tail). Then, small fibers located in the cell body can pull the tail of the cell towards the head, to move the cell forward, in a repetitive process of protrusion-adhesion-contraction-detachment. Different proteins present on the cell surface regulate cell movement by coordinating adhesion of the cells to the substrate. Our previous studies show that Thy-1/CD90, a protein found in neurons, activated endothelium and some fibroblasts, regulates cell movement through its interaction with two other membrane proteins: integrin and Syndecan-4. Here, we describe the mechanism by which Thy-1/CD90 controls such movement, by unveiling a novel interaction between Syndecan-4 and PAR-3, a protein that has been involved in cell polarity. When this interaction was blocked, for example after decreasing the amount of PAR-3 or Syndecan-4, we found that cells did not move, because they were not capable of disassembling those points of contact with the substrate, thus impeding the cyclic process of cell motility.

Background
Focal adhesion (FA), a key type of adhesive cell and extracellular matrix contact, is relevant for both mechanical and biochemical signaling [1]. The binding of transmembrane proteins (e.g., integrins and Syndecan-4) to extracellular ligands and the cytoskeleton mediates FA formation, thus promoting cell adhesion. A critical component in the cell-adhesion process is the adhesome -i.e., a complex of > 180 proteins and associated effector partners that connect with receptors such as integrins and Syndecan-4 [2,3] regulating not only cell adhesion but also other cellular processes, including cell polarization, and migration. Cell migration is a cyclical process that first requires cell polarization and the formation of membrane protrusions at the leading edge. Then, FA and actin microfilament bundles (i.e., stress fibers) form, and cells contract/retract the rear end to promote forward movement [4,5]. Many receptors and proteins are involved in cell movement, nevertheless; the key signaling components of the adhesome that regulate FA dynamics remain poorly defined.
An integrin and Syndecan-4 cellular counterpart possessing an integrin-binding site and a heparin-binding domain (HBD) is the neuronal surface protein Thy-1/CD90, a protein included in the integrin adhesome by virtue of the Thy-1/β3 integrin interaction described initially by our group [6,7]. Interestingly, Thy-1/CD90 HBD has also been involved in the formation of FA in astrocytes through the engagement of the proteoglycan Syndecan-4 in a cooperative interaction with αvβ3 integrin [8].
Syndecan-4 possesses a cytoplasmic domain with two conserved regions flanking a central variable region that binds to phosphatidylinositol 4,5-bisphosphate (PIP 2 ) [9][10][11] and PKCα [12][13][14] and this complex has been implicated in FA formation [15,16]. In fibroblasts, available evidence indicates that the small GTPase Rac1 localizes to the cell leading edge after stimulation with Fibronectin in a PKCα-dependent manner, indicating that Rac1 is the main GTPase activated downstream of Syndecan-4 ligation [13]. In our model of Thy-1/CD90induced astrocyte migration, Syndecan-4 engagement by Thy-1/CD90 activates FAK/Rac1, which are required to induce astrocyte polarization and migration over the extracellular matrix (ECM) [8,17]. However, the signaling partners of Syndecan-4 linking this proteoglycan to the FAK/Rac1 pathway are still unknown.
The cytoplasmic C2 domain of Syndecan-4 possesses an EFYA C-terminal sequence, which binds proteins containing the post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein (PDZ) domains such as Syntenin, CASK, synectin, synbindin and the Rac1 guanine nucleotide exchange factor (GEF) Tiam1 [18,19]. To learn more about other possible partners of Syndecan-4, here we performed mass spectrometry of complexes precipitated with Syndecan-4 cytoplasmic tail peptides and found the adaptor protein PAR-3 as a new binding partner for Syndecan-4.
Partitioning-defective (PAR) proteins play roles in the polarized cell migration of astrocytes, fibroblasts, and Tcells [20,21,27]. For example, the leading edge of motile cells present the PAR-6/aPKC polarization complex, which is needed to reorient the microtubule-organizing center, the microtubule cytoskeleton, and membrane trafficking towards the leading edge [22][23][24]. Particularly notable among the PAR proteins is PAR-3, a protein related to cell polarity in several cell types [23,25,26]. Most interestingly, PAR-3 possesses three PDZ domains and binds to Rac1 GEFs at the leading edge of cells, thereby spatiotemporally controlling Rac1 activity and cell migration [26,27]. While polarized cell migration clearly depends on PAR proteins, a possible role for PAR-3 as a downstream effector of Syndecan-4 has yet to be determined.
In adult individuals, mesenchymal cells are relatively quiescent cells that help maintain connective tissue and regulate homeostatic functions, such as stabilizing even the smallest blood vessels [28]. Tissue damage or inflammatory processes activate these cells, with fibroblasts transforming into myofibroblasts and astrocytes into reactive astrocytes [29]. These transformed cells are motile and scar-forming. Although an initially beneficial process, scarring requires fine-tuned regulations for appropriate tissue repair [30][31][32][33]. Thus, understanding and influencing the wound-healing process necessarily requires complete knowledge on the molecular mechanisms governing cell migration.
Here, we used astrocytes and fibroblasts to study the involvement of Syndecan-4 and PAR-3 in cell adhesion/ migration. The obtained research data identify a novel signaling pathway, termed Syndecan-4/PAR-3, involved in controlling Thy-1/CD90-induced FA dynamics in mesenchymal cells by regulating Focal Adhesion Kinase (FAK) autophosphorylation and Tiam1 activation. PAR-3 binding to Syndecan-4 further suggest that PAR-3 is a novel adhesome-associated component with an essential role in polarized cell migration.
To downregulate PAR-3 expression we used siRNA ON-TARGET plus SMART pool (Thermo Scientific) against rat PAR-3 or FlexiTube siRNA Mm_Pard3_4 (Qiagen) targeting mouse PAR-3. A mix of two Tiam1 siRNA targeting the human Tiam1 with 88% homology with the rat Tiam1 sequence (Dharmacon) was used to lower Tiam1 expression. Silencer® Negative Control No. 1 (Ambion™) or AllStars (Qiagen) were used as siRNA negative control. Plasmids and siRNAs transfections of DI TNC1 cells were performed with Amaxa Nucleofector following manufacturer's recommendations for astrocytes (Amaxa Biosystems, Lonza). Lipofectamine RNAiMAX Reagent and Lipofectamine 3000 (Thermo Fisher Scientific) were used to transfect MEFs.

Immunoprecipitation and Western blotting
DI TNC1 cells were serum-deprived for 16 h and stimulated or not for 30 min with a complex of Thy-1-Fc or TRAIL-R2-Fc conjugated to Protein A, or 3% FBS as a migration-inducing control. Then, cells were lysed (50 mM Hepes-KOH, pH 7.4, 50 mM NaCl, 750 μM CaCl 2 , 1 mM MgCl 2 , 1% Triton X-100, 2 mM DTT) supplemented with protease and phosphatase inhibitor cocktail (Biotool) and pre-incubated with streptavidin-coated Sepharose beads to clean lysates of biotin containing proteins. Soluble fraction of lysates was incubated with streptavidin-Sepharose beads coupled to biotinylated peptides (15 μg/sample) corresponding to 10 amino acids of the cytoplasmic tail of Syndecan-4 (K K A P T N E F Y A) or control peptides in which the last 4 amino acids were replaced by glycine (K K A P T N G G G G) [41]. Samples were separated by SDS-PAGE and silver stained. In-gel bands corresponding to Syndecan-4 tail-precipitated proteins that were absent in the control condition (modified peptides) were extracted from the gel, digested and analyzed by liquid chromatography-tandem mass spectrometry (UNC Michael Hooker Proteomics Center-UNC, Chapel Hill). Data, obtained from two independent experiments, were searched against publicly available databases using Mascot search engine. In a different set of experiments, precipitated proteins were immunoblotted for PAR-3 and Syntenin to corroborate mass spectrometry results.
For Syndecan-4 immunoprecipitation, cells were transfected with Syndecan-4-HA plasmid; 48 h later, cells were serum-starved for 16 h and stimulated for 30 min or not with Thy-1-Fc or TRAIL-R2-Fc conjugated to Protein A. Cells were lysed (20 mM Tris-HCl, pH 7.4, 0.15 N NaCl and 0.5% NP-40, supplemented with protease and phosphatase inhibitor cocktail) and incubated with a mouse anti-HA antibody. Precipitated proteins were analyzed by Western blotting for PAR-3, FAK, Syndecan-4 and Syntenin using rabbit polyclonal antibodies. Alternatively, MEFs were transfected with Syndecan-4-HA plasmid and selected with G418 (1.65 mg/ml). Cells were plated at sub-confluency, serum-starved for 16 h and stimulated with Thy-1-Fc-Protein A beads for 30 min or 3% FBS for 3 min. Cell lysates were incubated with rabbit anti-PAR-3 antibody and precipitated proteins were analyzed by Western blotting for HA (Syndecan-HA, mouse anti HA antibody) and Syntenin (rabbit antibody). An HRP-conjugated goat anti-rabbit IgG, Fc specific secondary antibody was used to detect the anti-Syntenin antibody and avoid interferences by the light chain immunoglobulin used to precipitate PAR-3. Antibody detection was performed using an enhanced ECL detection system (GE Healthcare). The intensity of the bands was quantified by using ImageJ (National Institutes of Health).

Proximity ligation assay (PLA)
DI TNC1 cells were transfected with Syndecan-4-HA or control plasmids. After 48 h, cells were serum-starved for 30 min and stimulated or not with Thy-1-Fc or TRAIL-R2-Fc conjugated to Protein A for 30 min. Cells were fixed and permeabilized with 0.1% Triton for 10 min. PLA was performed using DuoLink PLA probes and reagents (Sigma-Millipore) following the manufacturers protocol. Signal puncta was analyzed using the Analyze Particles function in ImageJ software as described below.

Indirect immunofluorescence
Cells were fixed at the indicated time points, rinsed, permeabilized and blocked as previously reported [8]. Cells were stained with appropriate antibody as described in each figure legend and coverslips were washed and mounted using 10% Mowiol-2.5% 1,4-Diazabicyclo [2.2.2] octane. Samples were visualized using a confocal Nikon Spectral C2 Plus microscope (Plan-Apo NA1.4 60x oil) or a Zeiss LSM 510 META Laser Scanning Confocal Microscope (Plan-Apo 420,782-9900 63x oil).

PAR-3 and Syndecan-4 immunofluorescence
DI TNC1 cells were transfected with Syndecan-4-HA and 15.000 cells were seeded on coverslips in 24-well plates for 24 h. Then, cells were starved for 30 min and stimulated or not with a complex Thy-1-Fc or TRAIL-R2-Fc (4 μg/well) [6,39] conjugated to Protein A in a 10: 1 ratio, for 60 min. Next, cells were processed by immunofluorescence as described [42].
Wound healing and polarity assays DI TNC1 cells were transfected and seeded in 24-well plates for 24 h at 70-80% confluence. For wound healing assays, cells were transfected with siRNA targeting PAR-3, Tiam1 or control, or with DN-Tiam1 (dominant negative for Tiam1), and for polarity assays, with siRNA targeting PAR-3 or siRNA control. DI TNC1 monolayers were scratched with a sterile micropipette tip; then, attached cells were washed twice with PBS and left in serum-free RPMI media for 30 min before the addition of the recombinant proteins to stimulate migration. Cells were stimulated for 7 (polarity) or 24 h (wound-healing) with Thy-1-Fc or TRAIL-R2-Fc conjugated to Protein A as described [43]. Since cells are kept in serum-free media during the stimulation period, no proliferation effect is foreseen, as we have previously shown [17]. The cell-free area was measured at time zero (before applying the stimulus) and at 24 h by phase contrast microscopy to quantify wound closure [43] using ImageJ software [44]. In cell polarization experiments, samples were fixed after stimulation and stained for nucleus (DAPI) and the Cis-medial Golgi protein giantin (rabbit anti-giantin from Covance Research Products). Cells were considered polarized when the Golgi signal was localized in front of the nucleus within an angle of 120°(see Fig. 2b). One hundred cells were monitored per condition, and cell polarization was evaluated as the percentage of cells along the wound border exhibiting polarized Golgi structures [45].

Far-indirect immunofluorescence for active GEFs
DI TNC1 cells were transfected with siRNA PAR-3 or siRNA control and seeded in 8-well Falcon culture slides (Corning) for 24 h at 50% confluence. Cells were serumstarved for 30 min and stimulated or not with Thy-1-Fc or TRAIL-R2-Fc conjugated to Protein A. Then, cells were subjected to a modified protocol of the original Far-immunofluorescence method previously described [46]. Briefly, cells were fixed, permeabilized and blocked as for regular immunofluorescence [43]. Samples were then incubated with GST-Rac1G15A or GST purified recombinant protein for 60 min and washed. Subsequently, samples were incubated with anti-GST antibody followed by secondary Alexa Fluor 546 goat anti-rabbit. GST-recombinant proteins were prepared according to the original report [47]. Immunofluorescence obtained with GST only was considered as background and subtracted from all samples. Similarly, in other experiments cells were stained for GST and Tiam to determine the levels of Tiam activation in situ in astrocytes. The colocalization between GST-Rac1G15A with Tiam was analyzed using ImageJ [44].

Focal adhesion analysis
DI TNC1 cells were transfected with siRNA PAR-3 or siRNA control. For video microscopy siRNAs were cotransfected with mCherry-vinculin. Cells were then seeded in coverslips in 24-well plates for assembly and disassembly assays, or in 8-well Lab-Tek II chambered coverglass (Nunc) for time-lapse video microscopy. For assembly assays, cells were serum-starved for 30 min and stimulated or not with Thy-1-Fc-Protein A or TRAIL-R2-Fc-Protein A for 15 min. For disassembly assays, cells were serum-starved during 30 min and treated with 10 μm Nocodazole (Sigma, Aldrich) for 4 h (to disrupt microtubules); then, Nocodazole was removed (time zero [t0]) promoting polymerization of microtubules and disassembly of FAs. The stimuli (Thy-1-Fc-Protein A or its control TRAIL-R2-Fc-Protein A) were applied for different time points (0-60 min) as indicated. Cells were fixed and immunofluorescence with anti-vinculin antibody was performed. For time-lapse video microscopy images were captured starting at time zero up to 30 min (every 1 min) in a temperature controlled chamber assembled in an epifluorescence Nikon Eclipse Ti microscope. Kinetics of focal adhesions was measured from these time-lapse experiments, scoring at least 10 mCherry-positive focal adhesions per experiment.
Similarly, experiments to follow FA dynamics in MEFs were performed as described above, but cells were cotransfected with siRNA targeting PAR-3 or siRNA control and siGlo RNAi (GE Dharmacon). Cells were then treated with 0,1 μm Nocodazole for 4 h and stimulated with Thy-1-Fc-Protein A or TRAIL-R2-Fc-Protein A as described for astrocytes.
Quantification of focal adhesion area and number was performed following previously described protocols [42]. The average number of FAs as well as the average area per FA was determined for at least 50 cells in each experimental condition using the ImageJ/Fiji program and the function 'Analyze particles' [44].

FAK autophosphorylation
Cells were transfected or not with siRNA PAR-3 or siRNA control and seeded in 24-well plates for 24 h at 50% confluence for DI TNC1. Cells were manipulated as described for FA disassembly assay (see above). The stimuli (Thy-1-Fc-Protein A or TRAIL-R2-Fc-Protein A) were applied for different time points (0-60 min). Then, cells were lysed in Laemmli buffer containing the protease and phosphatase inhibitor cocktail, analyzed by Western blot (see above) for p-FAK (Tyr397) and total FAK.

Tiam1 activity
DI TNC1 cells were grown in 10 cm plates, and Tiam1 activity was measured using an affinity precipitation assay previously described [48]. Briefly, cells were serum-starved for 16 h and subsequently stimulated with previously fixed CAD (shThy-1) or CAD (shLuc) cells as reported [39] for different periods of time. Thy-1/CD90 is highly expressed on the surface of CAD cells and this mode of activation, previously validated by our group, is used when a large amount of Thy-1-Fc protein is required to stimulate cells [17]. After stimulation, cells were washed and scraped in lysis buffer (25 mM HEPES, pH 7.4, 0.1 M NaCl, 1% NP40, 5 mM MgCl 2 , 10% glycerol, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml aprotinin and 1 mM sodium orthovanadate). Next, cell lysates were incubated with 30 μg of GST-Rac1G15A bound to GSH-agarose beads for 1 h at 4°C and mixed gently on a rocking shaker. After washing, beads were resuspended in Laemmli buffer. Samples were resolved by SDS-PAGE, transferred to nitrocellulose membrane, which was blocked with 5% fat-free milk in 0.1% Tween-PBS, and incubated with anti-Tiam1 (1:1000). Active Tiam1 was determined by Western blot analysis from the precipitated fraction and normalized to total protein (input).

Statistical analysis
All data are expressed as the mean ± standard error of mean (mean ± s.e.m.) from three independent experiments (n = 3). For each experimental condition at least 50 cells were evaluated per every independent experiment. Data were compared through non-parametric Mann-Whitney analysis, 1-way or 2-way ANOVA, accordingly. Significant differences (*) were established at P < 0.05.

PAR-3 interacts with Syndecan-4
The C-terminal PDZ-binding domain of Syndecan-4 (C2) associates with various PDZ-containing proteins or other molecules involved in cell migration [18]. Therefore, polarized cell migration was stimulated with fetal bovine serum (FBS) to identify C2-recruited Syndecan-4 binding proteins. The astrocyte cell line DI TNC1 was selected as a mesenchymal-type cell model based on prior use in studies related to cell adhesion and migration [6-8, 17, 43]. DI TNC1 cells were first stimulated with FBS, and lysates precipitated using streptavidin-Sepharose beads coupled to biotinylated Syndecan-4 peptides corresponding to the C-terminal last 10 amino acids of the cytoplasmic domain (Additional file 1A). As a control peptide, the same 10 amino acids with the last 4 amino acids (EFYA) replaced by glycine were used [41]. The associated proteins were separated in an SDS-PAGE gel and Silver stained (Additional file 1B). The bands of interest were cut from the gel and identified by mass spectrometry. Astrocytes stimulated with FBS presented various proteins binding to the Syndecan-4 cytoplasmic peptide. PAR-3 was one of the detected Syndecan-4 binding proteins, making this the first study to describe such an interaction (Table 1). Other proteins included Syntenin (AF248548) ( Table 1), a protein that serves as a good control due to its known Syndecan-4 C2 domain binding properties [49]. Syntenin directly interacts with the Syndecan-4 EFYA sequence, making it a perfect experimental control for the specificity of the biotinylated peptides used in the precipitation assays [18]. Noteworthy, because these experiments are performed adding excess of ectopic peptides (15 μg/sample), no competition between PAR-3 and Syntenin for the biotinylated peptides is foreseen.
To confirm PAR-3/Syndecan-4 binding, the obtained lysate precipitates were also analyzed through Western blotting with the anti-PAR-3 and anti-Syntenin antibodies. Full length PAR-3 (180 kDa) and Syntenin were detected when precipitating with wild-type but not control Syndecan-4 peptides in non-stimulated cells ( Fig. 1a and b). Cell stimulation with FBS or Thy-1-Fc (a fusion protein of the cell adhesion molecule Thy-1/CD90) increased Syntenin precipitation with the biotinylated wild-type peptide, but PAR-3 precipitation was unaffected or only slightly affected ( Fig. 1a and b). Similarly, when astrocytes transfected with hemagglutinin (HA)-tagged Syndecan-4 were used for Syndecan-4 immunoprecipitation, we observed a modest increase in full length PAR-3 co-precipitated in Thy-1-Fc-stimulated cells than in non-stimulated or cells treated with TRAIL-R2-Fc (Fig. 1c). As in previous studies [6,43], we used a non-related protein (TRAIL-R2) fused to a Fc fragment (TRAIL-R2-Fc) as a negative control of the fusion protein Thy-1-Fc. We have previously shown that TRAIL-R2-Fc neither binds to cell surface receptors nor elicits cellular responses [8,17,43,50]. Additionally, as part of the complex precipitating with Syndecan-4-HA, we found Syntenin and FAK, two proteins involved in cell migration and known to interact with PAR-3 [51] (Fig.  1c). Syndecan-4 was also detected as an immunoprecipitation control in these experiments. Furthermore, in situ Proximity Ligation Assay (PLA) showed that PAR-3 interacts very closely (< 40 nm) with Syndecan-4 in Syndecan-4-HA-transfected astrocytes (Fig. 1d). The abundance of this interaction was also significantly increased after Thy-1-Fc stimulation, but not in non-stimulated cells or cells treated with TRAIL-R2-Fc (Fig. 1e).
Altogether, these results indicate that PAR-3 binds to Syndecan-4 and that its interaction could be regulated to a certain extend during FBS-or Thy-1/CD90-induced cell migration.

PAR-3 is involved in Thy-1/CD90-induced cell adhesion and migration, but not polarization
Prior research reports indicate that neuronal Thy-1/ CD90 engages Syndecan-4 in a cooperative interaction with αvβ3 integrin to initially induce FA formation in astrocytes (~30 min). Sustained Thy-1/CD90 stimulation leads to Rac1 activation and astrocyte migration (> 60 min) [8,17]. Considering that PAR-3 and Syndecan-4 form a complex in astrocytes, termed herein as the Syndecan-4/PAR-3 signaling complex, and that the abundance of such complexes changes in Thy-1/CD90stimulated cells (Fig. 1d and e), the participation of PAR-3 in Thy-1/CD90-induced adhesion, polarization, and migration was explored. First, PAR-3 full length (180 kDa) as well as its isoforms (150 and 100 kDa) were silenced using a siRNA pool (Fig. 2a). Cell migration was subsequently monitored in a woundhealing assay after 24 h of treatment with Thy-1-Fc. Cells with silenced PAR-3 isoforms and stimulated with Thy-1-Fc evidenced inhibited wound closure compared with the respective siRNA controls (Fig. 2a).
Second, cell polarization was evaluated using the same technique as above, but wound-healing was monitored after 7 h of Thy-1-Fc treatment. Golgi/nuclei staining distinguished non-polarized from polarized cells (i.e., Golgi apparatus in front of the nucleus and facing the wound border within a 120°angle [45] (Fig. 2b, left  panel). PAR-3 silencing did not abolish Thy-1-Fc-induced Golgi polarization (Fig. 2b). This polarization process was also unaffected by PAR-3 silencing in nonstimulated and TRAIL-R2-Fc control cells (Fig. 2b).
Finally, cell adhesion was evaluated with a FA assembly assay, where an anti-vinculin antibody was employed to detect FAs by immunofluorescence. Our results show that PAR-3-silenced astrocytes stimulated for 30 min with Thy-1-Fc had fewer total FAs, but, unexpectedly, individual structures were larger and longer than in control cells (Fig. 2c). In contrast, Thy-1-Fc-stimulated astrocytes in siRNA control cells presented more and larger FAs than did non-stimulated and TRAIL-R2treated control cells (Fig. 2c).
These results show that PAR-3 participates in the signaling pathways required for adhesion and migration. However, in agreement with a previous report that used astrocytes stimulated with FBS [27], PAR-3 in our model system was not involved in Golgi polarization.

PAR-3 regulates FA disassembly and favors FAK dephosphorylation
Considering the surprising results indicating that larger and longer FAs were detected in PAR-3-knockdown cells, a possible role of PAR-3 in FA disassembly was evaluated. Cells were first treated with Nocodazole, a microtubuledepolymerizing drug [52] that blocks FA turnover [53,54].
Nocodazole was washed out and cells were immediately stimulated with Thy-1-Fc; FA disassembly was then analyzed at different time points. Through vinculin staining, Thy-1-Fc-stimulated astrocytes evidenced more rapid FA disassembly (i.e., fewer and smaller FAs 5 min post-Nocodazole washout; Fig. 3a) than non-stimulated and TRAIL-R2-treated controls (i.e., no change in FA quantity, smaller FAs 15 min post-Nocodazole washout; Fig. 3a).
After identifying these key components in FA disassembly, investigation was conducted on a possible underlying signal transduction mechanism. Our previous reports indicate that αvβ3 integrin and Syndecan-4 bind to Thy-1/ CD90 and trigger signaling events that include activation of FAK, and PI3K downstream of integrin activation [17]. Consequently, PIP 3 increases at the plasma membrane allowing translocation of a Rac1 GEF that leads to the activation of Rac1 and cell migration, events that also involve engagement of Syndecan-4 and the activation of PKCα (Fig. 3c) [8,17].
Additional reports also indicate a role for FAK in FA turnover, where Y397-FAK autophosphorylation is an important molecular marker for events linked to the control of FA dynamics. While higher phosphorylation levels are observed when FAs are stabilized, Y397 dephosphorylation is associated with FA disassembly [55].
Therefore, these results indicate that PAR-3 controls FA disassembly and that the presence of PAR-3, during this process, favors Y397-FAK dephosphorylation. a Immunoblotting for PAR-3 isoforms in cells transfected with PAR-3 siRNA compared to control siRNA. Actin was the loading control. Migration was evaluated using the wound-healing assay. Evaluated were DI TNC1 cells with silenced PAR-3 or control, stimulated or not (NS) with Thy-1-Fc-Protein A (Thy-1) or TRAIL-R2-Fc-Protein A (TRAIL). Pictures show overlapping images of wound closure at time zero (red) and after 24 h (green). Dotted line shows the wound-closure boundary. Graph shows wound closure in relative units. b DI TNC1 astrocytes, transfected as in (a), were stimulated with Thy-1-Fc-Protein A (Thy-1) or TRAIL-R2-Fc-Protein A (TRAIL) for 7 h. After fixation, cells were stained for nucleus (DAPI) and Golgi apparatus using an anti-giantin antibody. Golgi (red) positioning within an angle of 120°in front of the nucleus (blue), facing the wound, was considered polarized (1). Non-polarized cells are labelled as (2). Arrow shows the direction of migration. One hundred cells were monitored per condition. Graph shows the percentage of polarized cells. Scale bar = 10 μm. c DI TNC1 astrocytes, transfected as in (a), were stimulated for 15 min. FAs were visualized by immunofluorescence using an anti-vinculin antibody (red) and DAPI for nuclei (blue). Scale bar = 10 μm. Image analyses were performed with the Analyze Particles Tool in the Fiji image processing software [44]. Values in the graphs represent the mean ± s.e.m. from 3 independent experiments (n = 3) determined from at least 50 cells in each experimental condition per experiment. Significant differences are indicated as *P < 0.05 Values in the graphs represent the mean ± s.e.m. determined from at least 50 cells in each experimental condition per experiment. b DI TNC1 astrocytes co-transfected with mCherry-vinculin and siRNA (control or PAR-3) were treated with Nocodazole and washout. Cells were then stimulated as in (a), and a 30min time-lapse microscopy video was recorded. Values in the graphs represent the mean ± s.e.m. determined from scoring at least 10 mCherrypositive focal adhesions per experiment. c Model depicting the interaction of αvβ3 integrin and Syndecan-4 with Thy-1, which triggers signaling events that include activation of FAK and PI3K generating PIP 3 . An unidentified Rac1 GEF, is then recruited to the plasma membrane, where it activates Rac1 and leads to cell migration. d DI TNC1 astrocytes, transfected as in (a), were treated with Nocodazole and washout, and cells were stimulated for different time periods (0-15 min). Cell lysates were immunoblotted for p-FAK or total FAK. e Graph values are compared against the normalized time 0 and represent the average numbers obtained from the ratio of p-FAK/FAK densitometric data analysis. All graphs show the mean ± s.e.m. of n = 3. Significant differences compared to control conditions are indicated as *P < 0.05, **P < 0.01, and ***P < 0.001

PAR-3 regulates Tiam1 activation during Thy-1-induced migration
Our previous work showed that Thy-1/CD90 engagement of αvβ3 integrin and Syndecan-4 activates Rac1 in astrocyte migration via an unknown Rac1 GEF (Fig. 3c) [17]. To further understand how PAR-3 is involved during Thy-1-induced migration, we first evaluated if PAR-3 participates in the activation of Rac1 GEFs using a modified Far-indirect immunofluorescence method [46]. This method was modified by using a nucleotide-free recombinant Rac1 protein (GST-Rac1G15A), which strongly binds to active Rac1 GEFs [47]. Therefore, GST-Rac1G15A was added to interact with active Rac1 GEFs present in cells expressing endogenous or reduced PAR-3 levels. As a result, higher levels of active Rac1 GEFs were detected in Thy-1-Fc-stimulated, siRNAcontrol cells than in TRAIL-R2-Fc cells (Fig. 4a, left panels). In contrast, cells with reduced PAR-3 levels had very low levels of activated Rac1 GEFs despite Thy-1-Fc or TRAIL-R2-Fc treatment (Fig. 4a, right panels).
Since PAR-3 can bind the GEF Tiam1 to activate Rac1 [56], we hypothesized that most of the active Rac1 GEFs observed in the Fig. 4a after Thy-1 stimulation could be Tiam1. To test this hypothesis, astrocytes were transfected with Tiam1-specific siRNA or with a dominant negative form of Tiam1 and wound-healing assays were conducted. Tiam1 silencing or dominant-negative expression prevented the enhancing effect of Thy-1-Fc on cell migration, suggesting that Tiam1 is required for Thy-1-Fc-induced astrocyte migration (Fig. 4b). Next, Tiam1 activation was confirmed by an affinity precipitation assay [17,48]. Due to the amount of stimulus required to perform this assay, we used fixed CAD cells as a source of Thy-1/CD90 to stimulate astrocytes. This mode of activation has been previously validated by our group [17]. We found that CAD cells expressing Thy-1 on their surface induced Tiam1 activation in astrocytes after 30-60 min of treatment (Fig. 4c), which is in line with Tiam1 involvement in signaling pathways that promote astrocyte migration [17].
To prove that the active Rac1 GEF, identified in cells through GST-Rac1G15A binding, and Tiam1 coincide in cellular localization, we performed the same Far-indirect immunofluorescence experiment but, evaluated colocalization of anti-GST and anti-Tiam1 antibodies. More Tiam1 signal (red fluorescence) was detected at the plasma membrane of Thy-1-stimulated control cells (siCTRL) compared with TRAIL-R2-treated cells (Fig. 5, top panels, ±Thy-1-Fc), indicative of increased amount of active Tiam1 in cells stimulated with Thy-1/CD90. Importantly, the active Tiam1 signal coincided with the staining of the GST-Rac1G15A probe, suggesting that the active Rac1 GEF observed in Fig. 4a corresponds to Tiam1. Finally, we evaluated if Syndecan-4 was necessary for Tiam1 activation. To accomplish this, the same experiment was repeated in Syndecan-4-silenced cells. The co-localization between GST-Rac1G15A probe and Tiam1 observed in siCTRL cells does not occur when cells are depleted of Syndecan-4 (Fig. 5, bottom panels). Syndecan-4 silencing decreased cell spreading but did not affect cell viability (data not shown) in these experiments. These results agree with previous reports [8,57,58]. Therefore, these results suggest that active Tiam localizes to the plasma membrane in lamellipodia-like protrusions in a Thy-1/CD90-and Syndecan-4-dependent manner (Fig. 5, Zoom and heatmap boxes).
Altogether these results indicate that PAR-3 regulates cell migration by (i) associating with the adhesome protein Syndecan-4, (ii) favoring FAK dephosphorylation during FA disassembly, and (iii) regulating Tiam1 activation. Considering our previous report indicating the requirement of Rac1 activation for astrocyte migration [17], we suggest that Tiam1 is the missing Rac1 GEF that activates Rac1, downstream of integrin/Syndecan-4 engagement by Thy-1-Fc.

Syndecan-4/PAR-3 signaling participates in FA disassembly in fibroblasts
Once the existence of the Syndecan-4/PAR-3 complex was established, specifically in relation to controlling cell migration in astrocytes, the presence and functionality of this complex was tested in a different mesenchymal cell, namely mouse embryonic fibroblasts (MEFs). Unlike the employed astrocyte cell line, MEFs are primary cells immortalized and used for few passages only; therefore, they are a complementary mesenchymal cell model. PAR-3 was immunoprecipitated in MEFs expressing Syndecan-4-HA, and Syndecan-4 co-immunoprecipitation was detected by immunoblotting. Syndecan-4 interacted with PAR-3 in non-stimulated cells, as well as in Thy-1-Fc or FBS-stimulated cells (Fig. 6a). Of note, the amount of Syndecan-4 precipitated with PAR-3 was higher in Thy-1-Fc or FBS-stimulated cells (Fig. 6a) compared with non-stimulated cells. Similarly, Syntenin recruitment to immunoprecipitated Syndecan-4 was also induced by stimulation (Fig. 6a). These findings indicate that the Syndecan-4/PAR-3 complex exists in MEFs and that the amount of PAR-3 and Syndecan-4 complex formation increases after either Thy-1-Fc or FBS stimulation.
As previously shown for astrocytes, the total quantity and average individual area of FAs increased in control cells after Thy-1-Fc stimulation and in the absence of PAR-3 these FAs were larger (Fig. 2c). In fibroblasts, PAR-3 silencing (Fig. 6b) resulted in cells with a similar number of FAs after Thy-1-Fc stimulation (Fig. 6c and  d), but with significantly larger FAs in Thy-1-Fc-stimulated (3.47 μm 2 ; Fig. 6e) than control MEFs (2.6 μm 2 ; Fig.  6e). Fig. 4 Thy-1-induced astrocyte migration requires PAR-3-dependent Tiam1 activation. a PAR-3 silenced DI TNC1 astrocytes were stimulated with Thy-1-Fc-Protein A (Thy-1) or TRAIL-R2-Fc-Protein A (TRAIL) for 60 min. Cells were fixed, and active Rac1 GEF was detected by immnunofluorescence using GST-Rac1G15A and anti-GST (purple) and Phalloidin (green). The white rectangle indicates the zoomed area. The color map indicates levels of Rac1 GEF activation, going from 0 (blue) to 256 (white). Scale bar = 10 μm. b DI TNC1 cells transfected with siRNA control (siCTRL), siRNA Tiam1 (siTiam1), or dominant negative Tiam1 (Tiam1-DN). The Western blot shows levels of Tiam1 in siRNA-transfected cells. Migration was evaluated using the wound-healing assay, and cells were stimulated as in (a) for 24 h. Images show wound closure at time zero (green) and after 24 h (red). c DI TNC1 cells stimulated or not (NS) with endogenous Thy-1 from fixed CAD cells for different periods of time. Active Tiam1 was affinity-precipitated using GST-Rac1G15A coupled to GSH-agarose beads and immunoblotted for Tiam1. Numbers in the graphs represent the average fold-increase of Tiam1 activity normalized to total Tiam-1. All graphs show the mean ± s.e.m. of n = 3. Significant differences compared to control conditions are indicated as *P < 0.05 To further corroborate the effect of PAR-3 in MEFs during FA disassembly, MEFs with silenced PAR-3 were treated with Thy-1-Fc or TRAIL-R2-Fc immediately after Nocodazole washout, and FAs were analyzed. FA disassembly was accelerated in control cells stimulated with Thy-1-Fc (beginning at 2.5 min, peak at 5 min; Fig. 6f) compared to control cells treated with TRAIL-R2-Fc (beginning at 5 min; Fig. 6f). At 2.5 min in these control cells, FAs were less abundant and smaller in size in Thy-1-Fc-stimulated than in TRAIL-R2-Fc-treated MEFs (Fig. 6f, g and h). In line with the results obtained in astrocytes, this effect was not observed after silencing PAR-3, a condition in which FAs did not disassemble in cells treated either with Thy-1-Fc or TRAIL-R2-Fc, at least during the observation period (Fig. 6f, g and h).
Therefore, these results confirm that PAR-3 is needed for Thy-1-Fc-induced FA disassembly in MEFs. In addition to the aforementioned findings, this outcome demonstrates that Syndecan-4/PAR-3 binding, as part of the adhesome complex, is a common mechanism used by mesenchymal cells, not just astrocytes, to promote polarized cell migration.

Discussion
Fully discovering the signaling pathways that govern cell migration is essential for understanding important processes, such as scarring and wound-healing. Syndecan proteins and syndecan signaling partners play key roles in these processes. Nevertheless, the contributions of syndecan signaling partners to cell adhesion and migration are poorly characterized. This study is the first to report on the participation of Syndecan-4/PAR-3 signaling in FA disassembly in mesenchymal cells, specifically astrocytes and fibroblasts, two cell-types that play a key role in scar formation [31,32].
One novel research finding was that the adapter protein PAR-3 is an associated member of the adhesome and a partner for Syndecan-4. The EFYA sequence in the C-terminal region of syndecan proteins binds with PDZ domain-containing proteins [23,25,26]. Several syndecan-binding proteins containing PDZ domains, such as Syntenin, CASK, Synectin, Synbindin, and Tiam1, have been previously described. In particular, PAR-3 contains three PDZ domains [59]. These domains  . Syntenin and PAR-3 were immunoblotted as positive and an IP control, respectively. IgG was used as a negative IP control. b MEFs transfected with the siRNA control or PAR-3 were analyzed by Western blotting for PAR-3 expression. Tubulin was used as a loading control. c MEFs co-transfected with siGlo and siRNA control or PAR-3 were stimulated with Thy-1-Fc-Protein A (Thy-1) or TRAIL-R2-Fc-Protein A (TRAIL) for 15 min. FAs and nuclei were respectively visualized by vinculin staining (red) and DAPI (blue). Transfected cells were identified by the siGlo signal (green). Graphs show the mean ± s.e.m. for the number (d) and average area (e) of FAs from at least 50 cells. MEFs were transfected as in (c), incubated with Nocodazole and washout, and immediately stimulated as in (c) for different time periods. Graphs show the mean ± s.e.m. for the number (f) and average area (g) of FAs from at least 50 cells. Significant differences are indicated as *P < 0.05, **P < 0.01, and ***P < 0.001. (h) Cells were co-transfected with Paxillin-EGFP and siRNA (control or PAR-3). Shown are representative images for non-stimulated cells (t0) and cells stimulated with Thy-1-Fc-Protein A (Thy-1) or TRAIL-R2-Fc-Protein A (TRAIL) for 2.5 min (t2.5). Scale bar =10 μm allow PAR-3 to interact with several proteins, including cell-cortex proteins associated with the cytoskeleton [18,60]. The present study is the first to directly investigate the interaction between Syndecan-4 and PAR-3; moreover, we showed that its interaction depends on the EFYA sequence present in the Syndecan-4 tail. The obtained findings sustain that a Syndecan-4/PAR-3 interaction exists, thus providing new insights into the signaling mechanisms downstream of Syndecan-4 and into the role of PAR-3 in polarized cell migration. A noticeable feature of Syndecan-4 is that it signals forming oligomers [10], thus Syndecan-4 could bind independently to PAR-3, Syntenin, Tiam1, and FAK bringing together all these proteins in a signaling complex.
All PAR-3 spliced variants (i.e., 180 kDa, 150 kDa, and 100 kDa) have the three PDZ domains, so they can potentially bind Syndecan-4 through its EFYA sequence. Our work was centered on the PAR-3 full length protein since this contains all signaling modules described to play a role during cell polarization. However, we cannot disregard that there was a differential precipitation of the 150 and 100 kDa isoforms while using Syndecan-4-HA compared with biotinylated peptides. Moreover, the expression of those isoforms seems to vary in total lysates between cell types. Further experiments are necessary to determine if there are other domains either in Syndecan-4 or PAR-3 sequences that modulate the binding through the terminal EFYA sequence or if the signaling is affected when different PAR-3 isoforms bind Syndecan-4, or even if cell confluency regulates the expression and/or stability of PAR-3 splicing variants.
We also observed that Syndecan-4/PAR-3 binding tends to increase after FBS or Thy-1 stimulation during the precipitation assays, but this increase was significantly bigger in PLA assays, suggesting that the interaction between Syndecan-4/PAR-3 may be lost after the rough precipitation conditions compared with milder conditions during in situ assays.
Another important finding was that PAR-3 is involved in cell adhesion and migration, but not polarization of astrocytes. Dissecting these biological processes is relevant for two primary reasons. First, PAR-3 has a recognized role in cell polarization as part of the PAR complex formed by PAR-6/aPKC. This complex generates and maintains epithelial and neuronal cell polarity [25], in addition to regulating polarized cell migration in several species and cell lines [23,56,61,62]. However, the polarity complex of migrating astrocytes does not contain PAR-3 [27]. The latter results agree with ours in that PAR-3 silencing did not affect Golgi polarization. Second, Syndecan-4 localizes and regulates FA dynamics in cell adhesion and migration [36], and PAR-3 partially localizes to FAs, binding and activating FAK and PI3K to regulate adhesion and directional cell migration [21,51]. Notwithstanding, the exact role of PAR-3 in these events was previously unclear.
Adding to the prior results, this study also found that PAR-3 is required for FAK dephosphorylation occurring concomitant to FA disassembly. Itoh et al. reported that the PDZ domains of PAR-3 interact with the FERM domain of FAK regulating adhesioninduced FAK phosphorylation in HeLa cells [51]. On the other hand, FAK autophosphorylation on the Y397 residue is required for FA dynamics and migration [55,63]. These data consequently suggest that PAR-3 might play a key role in regulating the FAK phosphorylation turnover that occurs during FA assembly/disassembly. However, the spatiotemporal link of these events is unclear. We postulate that, as a result of Syndecan-4 association, PAR-3 is recruited to FAs to regulate FAK phosphorylation, and thus, FA turnover. This proposal is supported by the finding that i) FAK co-precipitates with PAR-3 and Syndecan-4, ii) the absence of PAR-3 leads to the formation of long and large FAs, and that iii) specifically, during FA disassembly the absence of PAR-3 prevents FAK dephosphorylation. The extensive list of PAR-3-binding partners includes phosphatases such as PTEN and SHP-2, both of which can subsequently dephosphorylate FAK [64][65][66]. However, the mechanism by which PAR-3 favors FAK dephosphorylation requires further investigation.
The current study further discovered that Syndecan-4/PAR-3 complex regulates Tiam1 activation in astrocytes. In the context of PAR-3, the small GTPase Rac1, can be regulated by the GEFs Tiam1 or TRIO [67]. Different research groups [56,68] showed that PAR-3 interacts with Tiam1 to mediate Rac1 activation in migrating cells. In turn, PAR-3 sequesters TRIO in neural crest cell-cell contacts, thereby inhibiting TRIOmediated Rac1 activation [67]. Related research data show that Thy-1/CD90 induces Rac1 activation to promote astrocyte migration [17], an effect that is inhibited by the Rac1 GEF inhibitor NSC23766. This inhibitor impairs the activity of both Tiam1 and TRIO [69]. A possible explanation is that Tiam1/TRIO might regulate Rac1 activity during astrocyte migration. The presently obtained data align with the proposed role for Tiam1 in astrocytes; however, none of the conducted experiments rule out a possible role for TRIO.
Another noteworthy finding of this study was that PAR-3 binds to Syndecan-4 and regulates FA disassembly in both astrocytes and embryonic fibroblasts. Minor differences were observed in FA dynamics between cell types that may be related to their own characteristics: MEFs basally produce less and smaller FA than astrocytes. Consequently, in MEFs lower Nocodazole concentrations are required to destabilize the MT network and FA disassembly occurs faster than in astrocytes. Similarly, since astrocytes FA are bigger, the effect of silencing PAR-3 on FA size may tend to make adhesions to overlap, making it difficult to detect differences in the number of FA. Moreover, PAR-3 localizes to different subcellular compartments, including the leading edge, FAs, and cell-cell contacts, as well as being more diffusely distributed in the cytoplasm [18,51,62]. PAR-3 localizes to cell-cell contacts in neural crest cells [67], epithelial cells [25,64,66,67], and fibroblast monolayers [52,62,70]. In neural crest cells (another mesenchymal cell type), PAR-3 controls contact inhibition of locomotion [67], while in epithelial cells, PAR-3 is required to establish apico-basal cell polarity [25]. Importantly, the participation of PAR-3 in FA disassembly is a novel and previously unappreciated role of this protein in cellular behavior, one that could explain the activation of Rac1 at the leading edge of cells with polarized migration. In a wound-healing assay of a neural crest cell monolayer, contact inhibition of locomotion polarizes cells to the wounded area. Considering the results presented by Mayor and co-workers [67], it is plausible to speculate that the role of PAR-3 differs between cell-cell contact sites and the leading edge in neural crest cell and other mesenchymal-like cells, such as fibroblasts and astrocytes. PAR-3 could bind the Rac1 GEF (i.e. TRIO) in cell-cell contacts, thereby inhibiting Rac1 through Rac1 GEF sequestration and, ultimately, polarizing the cells to the wound. In contrast, PAR-3 at the leading edge would bind to the Rac1 GEF (i.e. Tiam1) to activate Rac1 and promote membrane protrusions and polarized cell migration. Different associated partners in each location could account for varied functions, or the distinct PAR-3 isoforms could regulate each function. Analysis of these possibilities requires further studies.
Considering that Syndecan-4 regulates FAK autophosphorylation, the activation of RhoA and Rac1 GTPases, lamellipodia formation, and cell adhesion and migration [8,17,43]; additionally that, PAR-3 binds Tiam1 and FAK [51,56,67,68], together with the present findings that Syndecan-4 binds PAR-3 and PAR-3 participates in FA disassembly (which correlates with increased FAK dephosphorylation and Tiam1 activation), we propose an integrative, comprehensive model for the role of Syndecan-4/PAR-3 signaling in FA dynamics (Graphical Abstract). We postulate that the Syndecan-4/PAR-3 signaling complex recruits Tiam1 and FAK, possibly to promote FAK dephosphorylation and the localization and activation of Tiam1 near the plasma membrane. This model highlights the role of the Syndecan-4/PAR-3 complex and FAK in controlling FA dynamics. At the membrane, Tiam1 activates Rac1 and is implicated in cell migration [71], and whether it is also involved in FA dynamics by virtue of its association with the Syndecan-4/PAR-3 signaling complex or by binding to PIP 3 at the plasma membrane (Fig. 3c) is yet to be investigated.

Conclusions
In summary, our findings indicate that PAR-3 is a novel Syndecan-4-binding protein, and that the newly termed Syndecan-4/PAR-3 signaling complex participates in FA disassembly. Both observations are key findings for understanding the signaling pathways regulating the adhesion and migration of mesenchymal cells. This novel Syndecan-4/ PAR-3 signaling pathway provides new insights into the signaling mechanisms downstream of Syndecan-4 and the role PAR-3 plays in polarized cell migration. Due to the importance of syndecans in cytoskeletal regulation, migration, and cell-cell as well as cell-matrix interactions [36,72,73], future studies addressing the novel Syndecan-4/PAR-3 pathway in processes such as wound-healing and scarring should be of major interest in efforts to treat fatal chronic wounds.