Carbon dioxide and MAPK signalling: towards therapy for inflammation

Inflammation, although necessary to fight infections, becomes a threat when it exceeds the capability of the immune system to control it. In addition, inflammation is a cause and/or symptom of many different disorders, including metabolic, neurodegenerative, autoimmune and cardiovascular diseases. Comorbidities and advanced age are typical predictors of more severe cases of seasonal viral infection, with COVID-19 a clear example. The primary importance of mitogen-activated protein kinases (MAPKs) in the course of COVID-19 is evident in the mechanisms by which cells are infected with SARS-CoV-2; the cytokine storm that profoundly worsens a patient’s condition; the pathogenesis of diseases, such as diabetes, obesity, and hypertension, that contribute to a worsened prognosis; and post-COVID-19 complications, such as brain fog and thrombosis. An increasing number of reports have revealed that MAPKs are regulated by carbon dioxide (CO2); hence, we reviewed the literature to identify associations between CO2 and MAPKs and possible therapeutic benefits resulting from the elevation of CO2 levels. CO2 regulates key processes leading to and resulting from inflammation, and the therapeutic effects of CO2 (or bicarbonate, HCO3−) have been documented in all of the abovementioned comorbidities and complications of COVID-19 in which MAPKs play roles. The overlapping MAPK and CO2 signalling pathways in the contexts of allergy, apoptosis and cell survival, pulmonary oedema (alveolar fluid resorption), and mechanical ventilation–induced responses in lungs and related to mitochondria are also discussed. Video Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s12964-023-01306-x.


Introduction
The SARS-CoV-2 pandemic highlighted how insufficiently we clinically treat excessive inflammation.Although the specific mechanisms leading to the production of proinflammatory cytokines and the activation of immune system components, as well as the signalling and other effects of proinflammatory cytokines and chemokines, are relatively well understood, the interrelationships among these factors are largely unclear.Due to the many mechanisms common to various pathologies and pathogen infections, including signalling pathways leading to inflammation and activated in response to inflammation, it is worth evaluating lessons learned during the COVID-19 pandemic, making conclusions about the treatments provided, and continuing the intensive search for effective therapies for inflammation.
Mitogen-activated protein kinases (MAPKs) regulate cell proliferation, survival, differentiation, migration, and apoptosis; oncogenesis; and neurodegeneration [1][2][3][4][5].Signals from cellular receptors are transduced by MAPKs to a wide variety of effector proteins, including transcription factors, which regulate cell functions according to environmental conditions.In this review, we focus on three subfamilies of MAPKs, namely, c-Jun N-terminal kinases (JNKs), extracellular signal-regulated kinases 1 and 2 (ERK1/2) and p38 MAPKs, as they are key players in the regulation of inflammation and play important roles in signalling pathways critical to the course of SARS-CoV-2 infection.In response to a wide variety of chemical and biological agents, these MAPKs not only promote the production of reactive oxygen species (ROS) and proinflammatory cytokines, including interferongamma (IFN-γ), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) but also regulate cellular responses to a wide range of cytokines [6][7][8][9].
MAPKs have been proposed to be carbon dioxide (CO 2 ) sensors because select MAPKs have been shown to be highly regulated by CO 2 in vitro, in human cells and in plants [10][11][12][13].CO 2 -dependent regulation of MAPKs has been demonstrated in several animal cell types and tissues (Table 1), but the direct influence of CO 2 on MAPK activity has not been previously considered.The effects of CO 2 on MAPK activity are very dynamic and depend on the concentration of CO 2 and the duration of CO 2 exposure.Although the mechanisms of action underlying the effect of CO 2 on MAPK functions remain unclear, an emerging pattern indicates that inactive ERK1/2 and plant ERK-type MAPKs are activated, and the functions of all the activated MAPKs studied thus far have been inhibited by increased CO 2 levels (Figs. 1 and 2).Importantly, among several groups of proteins proposed to be CO 2 sensors, only MAPKs are common to all eukaryotes, with the other eukaryotic CO 2 sensors being taxon specific.
In the following parts of this paper, we highlight ERK1/2-dependent processes that are augmented by an increase in CO 2 concentration and the harmful effects triggered by MAPK signalling that can be inhibited by elevating CO 2 levels.We indicate possible or previously observed consequences of purposely increasing CO 2 levels in relation to various aspects of COVID-19 and the most common comorbidities in patients with COVID-19.Since the pathogenesis and therapy of COVID-19 is an extremely broad topic, including the roles of MAPKs and CO 2 in these contexts, we do not describe all the possible benefits of increasing CO 2 levels in COVID-19 therapy in this article, as these benefits have been discussed in other recently published papers [27][28][29][30].We focus primarily on the cooperation of the CO 2 -MAPK signalling module, because the functions of CO 2 and MAPK largely overlap (Table 2, Figs. 3 and 4), and it has recently been suggested that MAPKs may be CO 2 receptors [10].Due to text length limitations, we mainly emphasize the benefits of a transient increase in CO 2 levels.A broader view of hypercapnia can be found in many recent review papers [31][32][33].

Changes in CO 2 levels in the pathogenesis of COVID-19
CO 2 is an important component of pH regulatory mechanisms in biological systems.In an aqueous environment, dissolved CO 2 partially reacts with water to form H 2 CO 3 , which dissociates into HCO 3 − and H + .The balance between H 2 CO 3 and HCO 3 − levels underlies the most important mechanism of maintaining blood pH; H 2 CO 3 and HCO 3 − are able to neutralize excess bases and acids, respectively.Additionally, the bicarbonate buffer is highly efficient because the respiratory system efficiently removes CO 2 and dissolved inorganic carbon species are distributed on the basis of pH (Bjerrum plot, Fig. 5).Namely, increasing CO 2 levels leads to acidification and a shift in the CO 2 /HCO 3 − equilibrium towards a further increase in CO 2 levels and a decrease in the concentration of HCO 3

−
. These changes result an increased rate of CO 2 removal via the lungs and restoration of baseline pH and CO 2 /HCO 3 − levels.The transitions between CO 2 and HCO 3 − are catalysed by carbonic anhydrases (CAs), which play important roles in the regulation of pH and CO 2 levels [191].CAs, depending on the isoform, conditions, pH, and CO 2 /HCO 3 − ratio, may accelerate the hydration of CO 2 or catalyse the reaction in the opposite direction, thereby affecting the flow of CO 2 across cell membranes because CO 2 can be efficiently transported through membranes via diffusion, while HCO 3 − transport through membranes requires the action of transporters [192,193].
Numerous reports indicate that both HCO 3 − [194][195][196] and total CO 2 concentrations are lower in patients who die with COVID-19 than in patients who have recovered from COVID-19 [197][198][199].HCO 3 − levels lower than 22 mM have been identified as an important risk factor for mechanical ventilation [200] and a predictor of clinical deterioration in patients with nonsevere COVID-19 [201].The decrease in the total pool of CO 2 /HCO 3 − in COVID-19 patients is accompanied by an increased level of lactate.The decrease in CO 2 /HCO 3 The increased production of lactate compensates for failure to maintain normal pH.However, the primary cause of the increase in lactate may be an increase in the local glycolysis rate, which is a typical response to infection, inflammation and a decrease in oxygen supply [202].A reduced rate of aerobic respiration in conjunction with increased glycolytic lactate production leads not only to a decrease in mitochondrial CO 2 production but also to a decrease in pH.This acidification forces a shift in the CO 2 /HCO 3 − equilibrium, which increases the arterial partial pressure of CO 2 (PaCO 2 ), and elevated PaCO 2 accelerates the removal of CO 2 via the lungs, resulting in a reduction in the total pool of CO 2 /HCO 3 − .Since lactate, in contrast to CO 2 , cannot be removed easily via gas exchange, an increase in lactate can lead to a permanent decrease in pH and metabolic acidosis.Although metabolic acidosis does not typically occur in the course of acute COVID-19, blood lactate levels were the highest in nonsurvivors and were higher in hospitalized COVID-19 patients than in ambulatory patients [199,203,204].The greatest differences between a group of patients with COVID-19-related acute respiratory distress syndrome (ARDS) who presented with the "hyperinflammatory" phenotype, with a significantly higher mortality rate and a group of those who presented with the "hypoinflammatory" phenotype, was elevated lactate levels and decreased HCO 3 − levels in patients with the "hyperinflammatory" phenotype; the differences in other markers of inflammation were much less pronounced [205].
Similar to the effect of lactate, permissive and therapeutic hypercapnia leads to a decrease in pH due to an increase in PaCO 2 resulting from insufficient CO 2 removal through the lungs and the inhalation of CO 2 , respectively.However, hypercapnia-induced respiratory acidosis does not lead to the many complications attributed to metabolic acidosis and exerts a protective effect in patients with one of many other medical conditions [206][207][208][209].
In addition to studies demonstrating the ability of elevated CO 2 to inhibit the proinflammatory response induced by SARS-CoV-2 elements [10], there are also reports based on randomized trials and case studies showing the benefits of using HCO 3 − in experimental COVID-19 therapies to improve prognosis.Patients with mild COVID-19 who received 14-day nasal NaHCO 3 irrigation twice daily showed an eightfold lower risk of hospitalization than the overall population [210].Supplementing standard COVID-19 therapy with 8.4% NaHCO 3    [181, [185][186][187][188][189][190] steam inhalation led to an improvement in clinical parameters in patients with mild to moderate symptoms [211].A positive effect of 10 ml administration of 4.2% NaHCO 3 every 6 h was found on mechanically ventilated patients [212].

Opposing effects of elevated CO 2 levels
Although the physiological importance of CO 2 is well understood, its effects at the molecular and cellular levels are poorly understood, and the broad spectrum of CO 2 concentrations has almost never been compared in CO 2 signalling studies or for the therapeutic application of CO 2 .The different effects of specific CO 2 concentrations have been reflected in numerous seemingly contradictory results from research groups that reported results based on different CO 2 concentrations.Notably, the long-term effect of severe hypercapnia exerted the exact opposite effect of short-term CO 2 application, e.g., airway muscles were constricted after long-term (3 or 7 days of 10% CO 2 inhalation) treatment with CO 2 [213] and were dilated after short-term exposure to elevated CO 2 [67].CO 2 dilates airways that are constricted, e.g., by drugs such as serotonin, methacholine, bethanechol and carbachol, or by the occlusion of the pulmonary artery [68,69].Importantly, short-but not the long-term effects of hypercapnia are opposite those of hypocapnia, as transient hypercapnia dilates and hypocapnia constricts airways, as found in dog [70], porcine [71] and rat [72] models.In contrast to acute long-term hypercapnia, slightly elevated CO 2 levels exert a bronchodilator effect in healthy subjects and in patients with asthma before and after exercise [73,74].Consistent with the proposed inhibition of active ERK1/2 induced by elevated CO 2 , inhibition of ERK1/2 increased airway conductance in patients with asthma [75].
The abovementioned opposing effects of CO 2 at the physiological level are analogous to the opposite effects of CO 2 on ERK1/2 functions (the activation of inactive ERK1/2 and inhibition of activated ERK1/2).For example, in healthy subjects, elevated PaCO 2 levels increased pulmonary artery pressure [214], but inhaled 5% CO 2 reduced preexisting pulmonary artery hypertension (PAH) [77].Importantly, PAH has been associated with high levels of ERK1/2 and p38 activity [80].A direct comparison indicated that cyclic stretchinduced injury in human bronchial and alveolar epithelial cells was more efficiently inhibited by hypercapnia applied after cell stretching had begun than by preconditioning the cells via induced hypercapnic acidosis [53].The specific effects of CO 2 on MAPK activity and physiology are limited to a relatively narrow range Fig. 3 An overview of the involvement of MAPKs and the potential beneficial effects of elevated CO 2 levels on the pathogenesis of COVID-19 and comorbidities.Red arrows: confirmed impact of elevated CO 2 levels of CO 2 concentrations.For example, drastically elevated CO 2 (20%, PaCO 2 140 mm Hg) did not inhibit ERK1/2 activity [18] unlike lower CO 2 levels.Similarly, moderate hypercapnia (PaCO 2 of 80-100 mm Hg), conferred better protection from high-pressure ventilation-induced inflammatory injury on rat lungs than PaCO 2 > 100 mm Hg [54].
In summary, prolonged exposure to very high levels of CO 2 exerts detrimental effects on organisms.However, in the following sections of this review, we focus on the beneficial effects of CO 2 and its potential therapeutic use, specifically, the short-term effect of slightly elevated CO 2 concentrations.

The role of MAPKs in viral infections
MAPKs are activated by viral infection.For example, p38 is activated by hepatitis B virus (HBV), hepatitis C virus (HCV), influenza virus, enterovirus 71, human immunodeficiency virus (HIV) and dengue virus infection [215,216].Moreover, MAPKs are involved in many viral infection mechanisms.In addition to induction of a proinflammatory response and regulation of the activity of various types of immune cells during viral infections (e.g., the regulation of CD8 + T-cell apoptosis) [217], ERK, JNK and p38 isoforms have been shown to directly support viral multiplication.First, ERK1/2 may positively influence the entry of SARS-CoV-2 into host cells [218].Second, the phosphorylation of different host proteins by MAPKs facilitates the replication and translation of many viral proteins [219].Third, efficient nuclear export of viral ribonucleoprotein complexes depends on the activity of ERK1/2 (e.g., influenza virus ribonucleoproteins [220]), and fourth, phosphorylation of viral proteins by MAPKs facilitates viral complex assembly (e.g., p38α phosphorylates the HCV core protein, leading to its oligomerization [215]).Consist with these findings, p38 and ERK1/2 inhibitors impaired the replication of influenza virus and coronaviruses [221][222][223], including SARS-CoV-2 pseudoviruses, in an in vitro model [215].
Multiple mechanisms lead to the activation of MAPKs by SARS-CoV-2.One such mechanism is renin-angiotensin system (RAS) dysregulation, as SARS-CoV-2 causes internalization of ACE2 by inhibiting the primary function of ACE2, which is the cleavage of angiotensin (Ang) II to form Ang1-7.As a result, the production of Ang1-7 decreases, and the level of AngII increases, leading to the activation of AngII receptor type 1 (AT1) and downstream MAPKs.In addition to ACE2, other membrane receptors have been shown to interact with SARS-CoV-2 and trigger MAPK-mediated signalling; for example, spike protein activates p38 and ERK1/2 via the receptor CD147 in vivo [241] and in primary human cardiac pericytes [242], respectively.The role of MAPKs in SARS-CoV-2 signalling is multifaceted, as the activation of ERK1/2, JNKs and p38 MAPKs is also triggered by the SARS-CoV-2 nucleocapsid protein [243].
The importance of MAPKs has been confirmed not only in classic signalling studies involving MAPK inhibitors but also in many more-objective high-throughput analyses.Proteomic approaches clearly indicated that MAPKs have been found to be among the most highly activated proteins after SARS-CoV-2 infection, regardless of the experimental approaches, cell and sample types evaluated, or period of SARS-CoV-2 infection [237,[244][245][246]. RNA-seq data revealed that in addition to the regulation of MAPKs by SARS-CoV-2 at the transcriptional level in many cell types [247], the components of the MAPK signalling pathway were also strongly regulated via alternative polyadenylation sites in human peripheral blood mononuclear cells from COVID-19 patients [248].
All types of ACE2-positive immune cells, which are crucial for the pathogenesis of severe COVID-19, can be directly infected by SARS-CoV-2, and as a result of infection, activated MAPK signalling stimulates transcription factors such as NF-κB and AP-1, which trigger the production of proinflammatory cytokines.Despite disputes over whether endothelial cells can be infected by SARS-CoV-2, endothelial cells undoubtedly produce a strong proinflammatory response via MAPKs in patients with severe COVID-19 [249][250][251][252]. Vascular endothelial cells in infected organs recruit monocytes/macrophages and neutrophils to inflammation sites and promote further production of proinflammatory cytokines, leading to a cytokine storm [253,254].Uncontrolled activation of macrophages not only leads to the secretion of high levels of IFN-γ, IP-10, IL-6, IL-17, IL-10/23 and TNF-α but also causes a loss of inflammatory coordination mediated by type-I interferons, which is a hallmark of COVID-19.Importantly, type-I interferon production is inhibited by activated ERK1/2 in macrophages [255].In addition, MAPKs contribute to a decrease in lymphocyte counts, including lymphocyte necrosis and NK and T-cell exhaustion promoted by IL-6, which is commonly observed in COVID-19 patients [254].
In addition to regulating transcription factors, MAPKs regulate other types of effector proteins.For example, p38 and ERK1/2 phosphorylate and thereby increase the catalytic activity of a disintegrin and metalloprotease 17 (ADAM17).ADAM17, due to its proteolytic activity, can release the ectodomains of a variety of proteins; ADAM17 induces ACE2 shedding and the activation of proinflammatory cytokines and fibrotic factors, leading to enhanced organ dysfunction via increased inflammation and fibrosis [256].The roles of MAPKs in key processes in the pathogenesis of COVID-19 are very broad, as cellular responses to cytokines leading to severe disease in COVID-19 patients depend on MAPK signalling pathways.More detailed information on MAPK signalling in relation to SARS-CoV-2 infection can be found in recent comprehensive reviews [218,254,257].

Hypertension in COVID-19
Hypertension is one of the most common comorbidities that worsens the prognosis of COVID-19.The RAS plays a unique role in regulating blood pressure in patients with COVID-19 due to the direct effect of SARS-CoV-2 on ACE2.SARS-CoV-2 enhances the vasoconstrictive effect of AngII while reducing the amount of Ang1-7, which exert a vasodilating effect.MAPKs constitute a hub for these opposing activities, as both AngII and Ang1-7 signalling is mediated by MAPKs.p38 and ERK1/2 are activated in response to AngII binding by AT1 receptors and induce severe vasoconstriction, increasing blood pressure and heart rate.Moreover, MAPKs have been proposed to be sensors of pressure overload because activation of JNK, p38 and ERK1/2 is proportional to the amount of pressure overload and pressure overload-induced myocardial remodelling in hypertensive patients [258].In contrast to that of AngII, the activation of Ang1-7 signalling by Ang1-7 binding to the Mas1 receptor leads to inactivation of ERK1/2 via the induction of MAPK phosphatase-1 (MKP-1) in endothelial and vascular smooth muscle cells, which causes not only hypotension but also antiproliferative, antithrombotic and fibrotic effects [259][260][261].
p38 is overactive in the endothelium and adventitia of hypertensive model rodents in contrast to its activation level in normotensive animals.Activation of p38 in response to AngII activity was transient in normotensive rats but sustained in hypertensive rats [262].Progressive and sustained hypertension induced by AngII, a high-salt and high-fat diet, or monocrotaline treatment in model mice and rats was reversed by p38 inhibitors; similarly, endothelial dysfunction, vascular cell proliferation, cardiac hypertrophy, and enhanced extracellular matrix and collagen deposition leading to vascular remodelling were reversed [81,83].Inhibition of overactivated p38 thus prolonged survival and increased endothelium-dependent vascular relaxation [263].
ERK1/2 are activated in vascular smooth muscle cells, arteries and serum of hypertensive patients.ERK1/2 are crucial for AngII-and thrombin-induced smooth muscle cell proliferation and vascular remodelling, leading to hypertension, atherosclerosis, and accelerated cardiovascular damage [264,265].Similar to synthetic MAPK inhibitors, aerobic exercise exerts beneficial effects on vascular and endothelial functions, including the inhibition of vascular smooth muscle remodelling, which led to the acquisition of a hypertensive phenotype by promoting the inactivation of overactive p38 and ERK1/2 in spontaneously hypertensive rats [266].However, basal ERK1/2 activity has been shown to be essential for maintaining endothelial integrity in vivo, and ERK1/2 loss leads to rapid development of hypertension and death within 5 weeks due to widespread endothelial-to-mesenchymal transition and degradation of endothelial cells in various organs [267].
One of the arguments for the use of CO 2 in COVID-19 therapy is that the effect of CO 2 on the circulatory system is consistent with that of Ang1-7; increased CO 2 causes a decrease in vascular resistance and an increase in blood flow to organs [268,269].Experimental COVID-19 therapies based on various vasodilators have improved the prognosis.For example, sildenafil shortened the length of hospital stays and reduced the need for invasive mechanical ventilation [270].
In recent years, research on the effects of CO 2 on blood pressure has focused on the relationship between CO 2 levels and PAH, as end-tidal CO 2 (EtCO 2 ) is lower in patients with PAH than in control subjects, and PAH is associated with chronic alveolar hyperventilation.It has been shown that lower EtCO 2 or PaCO 2 results in shorter survival in patients with PAH [78,79].

Thrombosis
Inflammation of blood vessels in the lungs, heart, brain and other organs is the cause of the most serious complications of severe COVID-19, and COVID-19 is considered a form of inflammatory endotheliitis [249][250][251][252].Typical pathological changes include thrombosis, which may result from inflammation associated with the induction of tissue factor expression (i.e., factors whose mere presence triggers the production of blood clots).In cells that are in contact with platelets, including both monocytes and endothelial cells, the induction of tissue factor depends on p38.Accordingly, MAPK expression has been associated with platelet activation and thrombosis [88,89].However, the frequent incidence of thrombosis and the largeness of the thrombi in vessels of COVID-19 patients are largely due to neutrophil activity not inflammatory processes in endothelial cells.In response to SARS-CoV-2, nucleocapsid or spike proteins, IL-6 or, particularly, IL-8, neutrophils extrude neutrophil extracellular traps (NETs), a web of chromatin-based cytoplasmic materials enriched with antimicrobial agents.NETs promote the accumulation of activated platelets and coagulation factors, forming thrombi [90,[271][272][273].
More NETs have been found in deceased COVID-19 patients than in survivors, and there is a correlation between plasma NET levels and COVID-19 severity [91].
Notably, various studies have consistently shown that the greatest increase in NET production in COVID-19 patients occurs soon after admission to the intensive care unit (ICU).Independent research groups have reported that increased inspiratory airflow and mechanical cell stretch-induced MAPK and NF-κB activation in alveolar macrophages trigger the release of IL-8 and IL-6, which are crucial for NET induction [53,91,274].
Treatment with JNK, ERK1/2 or p38 inhibitors abrogates NET formation [90][91][92].Similarly, elevated CO 2 levels inhibit the processes that contribute to the formation of NETs, including bronchoalveolar fluid neutrophil infiltration, NF-κB activation, and the production of IL-6 and IL-8 [53,85].Moreover, the effect of CO 2 on the generation of NETs has been recognized.The formation of NETs is highly dependent on pH and the CO 2 /HCO 3 − ratio; specifically, NET production is induced by high pH and a low HCO 3 − level and inhibited by a high CO 2 level and low pH [86].
Assuming that the inhibition of MAPKs by CO 2 is a universal process, the antithrombotic role of CO 2 may be even broader than previously recognized, as thrombosis in patients with acute COVID-19 results from the binding of activated platelets to NETs, and MAPKs have been well documented in platelet activation in response to both SARS-CoV-2 and the spike protein [275].In addition, the role of CO 2 in reducing ROS production, as shown in peripheral occlusive arterial disease, points to a universal antithrombosis-inducing effect of CO 2 [57].Accordingly, acidosis promotes a reversible decrease in blood clotting [87].

Obesity and insulin resistance
Hyperinsulinaemia and hyperglycaemia induce an increase in the SARS-CoV-2 load [276], and high glucose levels lead to higher cytokine and ROS production [277] and activation of ERK1/2 in response to the spike protein in endothelial cells [10].MAPK-dependent modification of blood vessel development programs, including regulation of the expression of intercellular adhesion molecule-1 (ICAM-1), which is responsible for the recruitment of leukocytes to sites of inflammation [278], increases the risk of patients with type 2 diabetes, among others with vascular disease, experiencing more severe COVID-19 [279].The incidence of cardiovascular complications, including stroke, and death resulting from venous thromboembolism and pulmonary embolism is several fold higher in diabetic patients than in the general population [280,281].
MAPKs are involved in various mechanisms leading to obesity, insulin resistance and diabetes, including low-level systemic inflammation; ERK1/2, JNK and p38, which are activated by a high-fat diet, promote the infiltration of monocytes/macrophages into adipose tissue, proinflammatory signalling and dysregulation of immune responses [112,113].However, the central role of JNK in the core insulin signalling pathway is the mechanism best understood thus far.Activated JNK (activated, e.g., by hyperglycaemia, free fatty acids, cytokines or ER stress [101][102][103][104][105]) and ERK1/2 phosphorylate insulin receptor substrate-1 (IRS-1) and thus prevent signal transduction after insulin binding to the insulin receptor, leading to alterations in insulin action [106].The JNK regulation of metabolism is multidimensional.JNK1 knockout protected mice from IRS-1 serine phosphorylation, insulin resistance, fatty liver and diabetes [107,108].JNK isoforms are essential regulators in the transition between obesity and type-2 diabetes [104]; JNK1/2 promotes the development of insulin resistance and obesity, whereas JNK3 protects against excessive adiposity [282].JNK1/2 and p38 promote adipogenesis [283] and regulate lipogenesis, thermogenesis and the browning of white adipose tissue.One of the most important mechanisms among these processes is the modification of mitochondrial function by inhibition of the transcription factor peroxisome proliferator-activated receptor α (PPARα) and uncoupling protein 1 (UCP1) expression in response to, for example, ER stress or a high-fat diet [104,105,114,115].
Direct relationships between insulin resistance, ERK1/2 and p38 activity, and elevated CO 2 levels have been found in adipocytes, which become insulin resistant as a result of postsurgical trauma.ERK1/2 and p38 are highly activated after surgery.Overnight incubation of adipocytes in 10% CO 2 inactivated ERK1/2 and p38 and restored insulin receptor and IRS-1 sensitivity to insulin [21].Consistent with CO 2 -dependent MAPK regulation, subcutaneous injections of CO 2 reduced body fat [109].In addition to the use of transcutaneous CO 2 as carboxytherapy in aesthetic medicine [110], the transcutaneous application of CO 2 is an efficient treatment for chronic diabetic wounds [284].Importantly, the major obstacle to wound healing is excessive neutrophil apoptosis caused by the production of NETs [285]; therefore, the beneficial effects of CO 2 on diabetic wound healing are consistent with the anti-NETosis effect of CO 2 , acidification and MAPK inhibitors [86,[90][91][92].

Stroke and ischeamia
Multiple organ failure is the leading cause of COVID-19 mortality.It is a result of, among other causes, organ hypoxia, including hypoxia due to ischaemic stroke, which is a frequent complication of COVID-19.One of the changes observed in the brain in acute stroke patients is a profound reduction in CO 2 level [286].
In addition to ischaemia, further organ damage is caused by the restoration of blood flow to organs; this damage is known as ischaemia-reperfusion injury.MAPKs in the brain are activated both in response to hypoxia and several minutes after reperfusion.MAPK inhibition has been shown to ameliorate brain injury due to reduced proinflammatory signalling and cell death.Decreasing MAPK activity enhances myelin regeneration, increases blood-brain barrier function, and suppresses inflammation [97].Many different drugs that exert neuroprotective effects during cerebral ischaemia inhibit MAPKs and downstream responses, including impairment of IL-1β, IL-6 and TNF-α production by purpurin and the inhibition of apoptosis by tetramethyl pyrazine mediated via JNK inactivation [98][99][100].Therefore, blocking the proinflammatory response by inhibiting NF-κB-dependent gene transcription in neurons leads to a reduction in the number of damaged neurons and even to their recovery, reducing the infarct area and death rate of neurons [287].
Therapeutic hypercapnia exerts broadly understood protective effects against reperfusion and oxidative brain injury after ischaemic stroke [136,286,288,289].In addition to cerebral vasodilation, therapeutic CO 2 reduces blood-brain barrier damage [289] and increases sensorimotor activity and spatial memory after focal cerebral ischaemia-reperfusion [136].In hypoxic regions, pH is lowered to be as low as 6.0-6.5, which confers neuroprotection [206,209,290].An increase in the concentration of protons may result in energy benefits; i.e., an increase in the number of protons contributes to maintenance of a higher membrane potential in the mitochondria, which leads to more efficient ATP production under conditions of oxygen deficiency.This protection has been demonstrated in fish [291], neurons [292] and neuroendocrine prostate cancer cells, in which acidic pH shifted cellular metabolism towards oxidative phosphorylation [293].This phenomenon may explain the protective role of CO 2 under conditions of hypoxia and the reduction in oxygen consumption by the brain when CO 2 levels are increased [184].
Studies on animal models of ischaemic injury have shown that hypercapnic acidosis exerts a protective effect not only on the central nervous system but also on the lung, myocardium, intestine and liver [34,[93][94][95].For example, an EtCO 2 higher than 20 mm Hg at intubation and its increase after resuscitation reduces neurological damage in patients after cardiac arrest [96].
There are known direct relationships between CO 2 and MAPKs in protection against ischaemia-reperfusion-induced injury; for example, the protective role of elevated CO 2 levels in the context of ischaemia-reperfusion-induced retinal injury is mediated via the inhibition of activated p38 [23].Hypoxia-induced ERK1/2 activity in mice was suppressed by 10 min percutaneous administration of CO 2 once per day, which was accompanied a CO 2 -induced increase in ischaemic blood flow and capillary density [19].

MAPKs and CO 2 levels in response to mechanical ventilation
Permissive hypercapnia is included in the treatment guidelines for intubated COVID-19 patients in most countries and institutions worldwide.A comparison of hypercapnic and normocapnic COVID-19 patients (PaCO 2 of 47.1 vs. 39.7 mm Hg, respectively) showed no difference in mortality despite worsened health in the hypercapnic patients; i.e., these patients had a higher body mass index (BMI) and higher number of venous thromboembolic events, chronic obstructive pulmonary disease (COPD) or ARDS [294].
In addition to the effect of hypercapnia on patients with COVID-19, there is debate as to whether hypercapnia should be used for intubated patients, as contradictory results have been obtained from different studies [295].In addition to reports showing the benefits of hypercapnia, there are studies showing reduced survival times for mechanically ventilated patients with severe hypercapnic acidosis [296,297].However, a recent meta-analyses indicated that permissive hypercapnia was associated with lower mortality than imposed hypercapnia under protective ventilation conditions [298].To date, no studies have been conducted in mechanically ventilated patients with a transient increase in CO 2 levels (that is, a level sufficient to inhibit the excessive activity of MAPKs in cultured cells [10], corresponding to 5% CO 2 administered for 15-20 min several times a day).It should be emphasized that CO 2 is a strong inhibitor of both innate and adaptive immune responses, including inhibition of lymphocyte and natural killer cell cytotoxicity, neutrophil and macrophage migration to sites of infection, and the release of proinflammatory cytokines; therefore, the prolonged use of elevated levels of CO 2 may lead to weakened immune protection against bacterial infections and sepsis [31,85], which may worsen the outcome for ICU patients.
The cytokine storm induced by infection with SARS-CoV-2 is enhanced by mechanical ventilation in patients with severe COVID-19.High-pressure mechanical cell stretching exacerbates lung injury, by changing cell histology, increasing lung infiltration with neutrophils, and inducing AEC apoptosis associated with caspase-3 activation [24].MAPKs are involved in the mechanisms leading to all of these adverse effects in lungs; the most widely recognized of these mechanisms is the role of ERK1/2, which lead to the downstream activation of NF-κB [53], ICAM-1 [54] or ADAM17 [20].Activation of these signalling pathways as well as ventilation-induced lung injury, AEC apoptosis and increased neutrophil infiltration can be reduced by either elevating the CO 2 level or inhibiting MAPK function [20,24,53,54,56].
Since high levels of oxygen support are used in mechanically ventilated patients with severe COVID-19, it seems that there may be additional benefits from the use of elevated CO 2 levels in these patients, as hyperoxia induces profound lung injury, AEC apoptosis, and ROS and proinflammatory cytokine production [59-66, 299, 300], and CO 2 inhibits the production of ROS and stimulates the production of antioxidants [57,58].

MAPKs and CO 2 regulate the resorption of alveolar fluid
Symptoms such as shortness of breath, a low SpO 2 level and lung failure, as well as organ failure, are largely due to oedema fluid (alveolar lining fluid, ALF) flooding alveolar spaces.Excess ALF markedly reduces the amount of oxygen delivered to erythrocytes.In healthy lungs, membrane transporters trigger vectorial ion transport, followed by osmotic influx water, from the apical surface to the basolateral surface of alveolar epithelial cells (AECs), i.e., from the lumina of the alveoli into the lung interstitium and endothelium.Failure of alveolar fluid clearance (AFC) results from a decrease in the level of membrane transporters needed for the flow of ions.AFC is lowered under hypoxic conditions [301], hyperoxic conditions [302,303], elevated airway pressure [304], pathogen infection and high levels of proinflammatory cytokines, including IL-1β, IL-8, TNF-α and transforming growth factor β1 (TGF-β1), in ALF [38,[301][302][303][304].
The main mechanism underlying AFC is the transport of Na + ions across the apical membranes of AECs via epithelial Na + channels (ENaCs).Activated ERK1/2 in AECs phosphorylate the β and γ subunits of ENaC, leading to enhanced interaction of ENaC with Nedd4, an E3 ubiquitin ligase, and to the endocytosis of the ENaC complex and subsequent downregulation [11,[39][40][41][42]. Thus, Na + ions accumulate in ALF, leading to an increase in its pH and volume [305].In addition, ERK1/2 have been indicated to be upstream regulators in the pathway leading to increased degradation of ENaC due to the phosphorylation of Nedd4 by JNK [11].Inhibition of ERK1/2 or JNK restores the stability of ENaC in the cell membrane, resulting in an increase in AFC.Moreover, in response to IL-1β, p38 inhibits the activity of the α-ENaC gene promoter and the trafficking of ENaC to the apical membranes of type II AECs (ATII) [38].After absorption into alveoli, Na + ions are eliminated through the basolateral side of AECs mainly via the action of Na/K-ATPase.Both activated ERK1/2 [12] and JNK [25,26] inhibit Na/K-ATPase, reducing the AFC rate.
In the studies on ENaC and Na/K-ATPase presented above, elevated CO 2 levels (60-120 mm Hg vs. 40 mm Hg of the control) inhibited AFC but were applied under control conditions to activate MAPKs [11,12,25,26,42].Under control conditions, inactive ERK1/2 were activated by elevated CO 2 levels; therefore, the reduction in AFC rate induced by an increase in CO 2 level was expected.However, elevating CO 2 levels shows potential therapeutic value for use under pathological conditions in which excessive ALF production and AFC impairment are observed as a result of high ERK1/2 activity stimulated by infection or inflammation.As active ERK1/2 are inactivated by a transient increase in CO 2 concentration [10], the therapeutic transient elevation of CO 2 in the lungs might lead to an increase in AFC under pathological conditions with elevated MAPK activity.These hypotheses are supported, at least in part, by reports showing that transient (20 min) hypercapnia (10% CO 2 ) increased AFC when ALF production induced by forskolin was also increased [35].
Moreover, AFC regulation by MAPKs is mediated by aquaporin channels (AQPs), through which water flows following an osmotic gradient.The expression of a key aquaporin, AQP5, is downregulated by p38 and JNK, e.g., in human SPC-A1 cells [43] or murine lungs [44].Generally, events or factors that activate ERK1/2, p38 and JNK (e.g., infection or cytokines) lead to a decrease in the expression of membrane ion or water transporters and an increase in pulmonary oedema via the action of MAPKs.Consistently, research on inflammation induced by pre-B-cell colony-enhancing factor (PBEF) has shown that ERK1/2 downregulated the main transporters responsible for AFC, i.e., ENaC, Na/K-ATPase, and AQP1 [45].
The commonly held view is that the beneficial therapeutic effect of NaHCO 3 is due to elevated pH.However, there should be no long-term changes in pH in the lungs after inhalation of NaHCO 3 , as any elevation in pH should be rapidly neutralized by Na + influx into the cytoplasm of AECs via ENaC and across the basolateral membrane into bodily fluids.Otherwise, the increased Na + concentration would be followed by increased secretion of ALF with all the associated negative consequences.In addition, the pH of ALF is regulated by paracellular HCO 3 − flux across the airway epithelium.At the correct (i.e., slightly acidic) pH of ALF, HCO 3 − is secreted.In contrast, when the ALF pH is increased (e.g., in response to infection or proinflammatory cytokines, and presumably after NaHCO 3 inhalation), HCO 3 − flow is reversed, limiting pH changes [305][306][307].These arguments may support the effect of elevated CO 2 but not an increase in pH in the alveolar epithelium via the therapeutic use of NaHCO 3 .
Among the most widely used and most effective (though still insufficient) drugs in the treatment of acute COVID-19 is highly concentrated dexamethasone, a corticosteroid that inhibits the activity of MAPKs [308].In addition to its anti-inflammatory effect, dexamethasone increases the amount of ENaC in AECs by inhibiting ERK1/2 [46,47] and increases the AFC rate.However, under many pathological conditions where ERK1/2 are activated, decreased sensitivity to glucocorticoids is observed [309,310].The molecular mechanism underlying the dexamethasone-dependent regulation of MAPKs is the upregulation of MKP-1 and, as a result, increased inactivation of MAPKs.Transcriptomic data indicate that SARS-CoV-2 infection leads to downregulation of MKP-1, reducing cell sensitivity to corticosteroids [311,312].Consequently, in contrast to elevated CO 2 , dexamethasone was not able to block the activity of ERK1/2 induced by the spike protein in bronchial epithelial cells in the presence of IFN-γ and TNF-α [10].
Because of the participation of mast cells in the course of COVID-19, antihistamines are among the most commonly prescribed medications in COVID-19 therapy.The importance of H1 receptor antagonists has been confirmed not only in numerous in silico and in vitro studies but also through its use in the clinic to alleviate the symptoms of COVID-19 in patients.Information on various H1 receptor modulators in the treatment of COVID-19 can be found in a recent review article [314].In addition, competitive inhibitors of histamine H2 receptors, such as famotidine, are very effective in relieving mild COVID-19 symptoms [315] and in protecting against death and intubation [124,316], although the exact molecular mechanisms are yet to be elucidated.
The inhibition of overactive MAPKs by elevated CO 2 levels in allergic reactions is of particular interest because MAPK/NF-κB-inhibiting drugs (e.g., lidocaine or p38 inhibitors) [129] and elevated CO 2 levels are both effective in treating allergy symptoms.In various clinical trials, noninhaled 100% CO 2 (flow rate 5-10 ml/s) was effective in the treatment of allergic rhinitis [116].The effect of a single dose of CO 2 administered intranasally for 10-30 s lasted 4 to 6 h, and a 60-s dose lasted 24 h.Similarly, after a 20-s exposure to CO 2 prior to allergen exposure, the acute responses to allergen challenge were reduced; for example, there were a significant reduction in sneezing, secretion weight and bilateral rhinorrhoea symptoms.CO 2 also led to inhibited histamine release [117].Accordingly, CO 2 inhibited mast cell degranulation and histamine release in vitro [118].Moreover, a decrease in PaCO 2 is one of the most common initial symptoms of anaphylactic reactions [119], suggesting the benefits of using elevated CO 2 levels for inhibiting the most severe allergic reactions.Taken together, the evidence suggests that the regulation of CO 2 -MAPK pathways in the inhibition of allergic reactions is a promising direction for future research.

COVID-19 and smoking
Early in the COVID-19 pandemic, controversial analyses indicated that, contrary to predictions, smoking did not only not worsen the prognosis but also may have protected patients against the development of severe COVID-19 symptoms [317][318][319].Various studies have pointed to a lower rate of daily smokers presenting with symptomatic COVID-19 [319] and a lower risk of hospitalization, serious illness or death compared to the general population [320][321][322][323][324][325][326].Interestingly, even in studies that concluded that smoking worsened the prognosis of COVID-19, the proportion of smokers with severe COVID-19 compared to the proportion of smokers in the general population showed that smoking conferred a protective effect [327].
Although the reasons for the potential protective effects of tobacco smoke are unknown, reports of the beneficial effects of smoking have been increasing, so clinical trials have been launched based on the hypothesis that nicotine plays a protective role against the development of COVID-19.Although the arguments presented in this paper may suggest that the effects of smoking considered to be positive may be due to the inhalation of elevated levels of CO 2 during smoking; however, this hypothesis should be considered with caution.Notably, as smokers have a much higher risk of cardiovascular and respiratory disease, their milder COVID-19 cases may have be due to the protective effects of the medications they take.On the other hand, numerous comorbidities in this group of patients, compared to the general population, may suggest a very strong protective effect of inhaled tobacco smoke.

MAPKs and CO 2 in breathing regulation
Shortness of breath is a typical symptom caused by infection with early variants of SARS-CoV-2; thus, the regulation of breathing plays an important role in the pathogenesis of COVID-19.During wakefulness, CO 2 levels are sensed mainly by central chemoreceptors, i.e., the chemosensory neurons in the medulla oblongata sensitive to CO 2 , which also sense a decrease in pH of cerebrospinal fluid, and carotid body chemoreceptors determine the sensitivity of central chemoreceptors to CO 2 [131,132].An elevated CO 2 level is the primary factor for increasing ventilation and blood flow to the brain.However, prolonged hypercapnia reduces the sensitivity of chemoreceptors to CO 2 , leading to slower of CO 2 -induced rapid breathing over time.Similarly, breathing becomes faster as the concentration of inspired CO 2 increases up to 9-10%, and a further increase in CO 2 concentration leads to a decrease in ventilation.
The mechanics of breathing regulation, especially, CO 2 level sensing, are very poorly understood at the molecular level.Mitochondria appear to be crucial for the response to elevated CO 2 , as CO 2 induces the immediate release of ATP from chemosensitive regions of the ventral surface of the medulla oblongata [133].ATP release is mediated by connexin 26 [328] and potentiates the release of acetylcholine [329].Studies with model animals indicated that ERK1/2 were crucial for the regulation of respiration, as inhibition of ERK1/2 in brainstem preparations led to impaired breathing responses to CO 2 -induced acidosis [134].
One of the most important goals in the treatment of severe COVID-19 is an increase in low SpO 2 levels.Brief inhalation of CO 2 increases SpO 2 [184].The increase in SpO 2 was immediate, within ~ 1 min, and 2-min sessions of inhaled 4, 8, or 12% CO 2 with nebulized perflubron, a synthetic surfactant, caused SpO 2 to increase by 1.7, 1.9 and 2.3 percentage points, respectively.The increase in SpO 2 was maintained for 20 min, and subsequent inhalation treatments (twice per day) further stabilized the patient, suggesting a cumulative beneficial effect.Statistically significant increases in SpO 2 in patients with cystic fibrosis are maintained 9 days after completion of the 5-day series of CO 2 inhalation [330].In another trial, inhalation of 8% CO 2 increased SpO 2 in subjects with mild allergic asthma after allergen-induced bronchoconstriction [331].
In addition to increasing SpO 2 , inhaled CO 2 increase the supply of oxygen to tissues because CO 2 allows oxygen to be released from haemoglobin (the Bohr effect) [332][333][334].Thus, the CO 2 inhalation treatment led to simultaneous increases in SpO 2 , better utilization of the oxygen in tissues because of the Bohr effect, and increases in blood flow due to vasodilation, which may support the use of less intense oxygen therapy.

CO 2 and sleep
Sleep disturbances are common symptoms of both COVID-19 and post-COVID syndrome.Changes in CO 2 levels are associated with sleep onset, wakening, and sleep stages.During sleep, PaCO 2 is typically 2-8 mm Hg higher than it is during waking hours, depending on the sleep stage, and the sensitivity of the medulla oblongata chemosensors to CO 2 decreases and hypoventilation occurs [154,155].Thus, an increase in PaCO 2 of 2-8 mm Hg in mechanically ventilated patients may not be considered hypercapnic but a desirable baseline physiological level.
The intensity of neuromotor responses regulating breathing is significantly reduced during sleep compared to that during wakefulness; therefore, only marked increase in hypoxemia or hypercapnia increase ventilation during sleep.Similarly, waking up may be triggered only by a decrease in SpO 2 to 70% or an increase in PaCO 2 by 15 mm Hg compared to eupnoeic levels.In contrast, the physiological tolerance for decreased PaCO 2 during sleep is low, since a decrease in PaCO 2 by 3-6 mm Hg during sleep leads to sleep apnoea.Therefore, eupnoeic PaCO 2 when awake may not be enough to sustain eupnoeic breathing during sleep [156,157].
Maintaining waking PaCO 2 leads to long-term sleep deprivation in patients in a medically induced coma.In mechanically ventilated patients, REM sleep is absent (and markedly reduced in patients with noninvasive mechanical ventilation) [335][336][337].REM sleep is associated with an additional increase in PaCO 2 of 1-2 mm Hg [158], local increases in low-frequency oscillations and global decreases in high-frequency oscillations in the electroencephalography (EEG) spectrum [338].CO 2 is the determining factor for changes in brain activity; inhaled CO 2 leads to an increase in low-frequency power in the EEG spectrum [184].There is a close connection between ERK1/2 and CO 2 in the regulation of REM sleep; active ERK1/2-brain-derived neurotrophic factor (BDNF) signalling in the pedunculopontine tegmentum promotes homeostatic control of REM sleep [339].Neurotransmitters involved in the regulation of sleep, the circadian rhythm and treatments that prevent major depressive disorder activate the key transcription factor cAMP response element-binding (CREB) via ERK1/2 signalling [159][160][161].
In mechanically ventilated patients, CO 2 supplementation is particularly beneficial because mechanical ventilation decreases both pO 2 and PaCO 2 in the lungs.Inhalation of 1.5-2% CO 2 is required to maintain the target EtCO 2 of 4.7-4.9% in mechanically ventilated patients [340].Interestingly, studies in model animals have indicated that the decrease in pO 2 and PaCO 2 that occurs in mechanically ventilated lungs can be inhibited by ERK1/2, p38 and JNK inhibitors [341].

Regulation of memory by MAPKs, CO 2 and mitochondria
The levels of cellular CO 2 produced via aerobic oxidation of carbohydrates are higher than those produced via other ATP synthesis pathways.Therefore, CO 2 signalling particularly affects organs that consume carbohydrates as their main sources of energy.Therefore, CO 2 is an important regulator of brain function, and as much as 20% of all CO 2 in the body is generated in the brain, even though the brain represents approximately 2% of human body weight.Hypocapnia occurs in 74% of individuals post-COVID, and patients show neurological symptoms: fatigue, insomnia, depression and post-COVID brain fog [342].Hypocapnia triggers known negative effects in various neurological diseases and conditions, such as severe traumatic brain injury, and ischaemic or haemorrhagic strokes.Moreover, hypocapnia negatively affects neonatal brain development, and the harmful effects can be reversed by inhaling 5% CO 2 [343].CO 2 inhalation enhances the formation of memories and long-term memory [136][137][138][139].The role of CO 2 in the regulation of memory has been indirectly discerned from numerous studies on CAs.CA inhibitors (e.g., acetazolamide) impair [140], and administration of CA activators enhances [141,142] memory and learning in model animals.
Notably, the connections between ERK1/2 and mitochondria, which are sites of CO 2 production, are important because endogenous CO 2 is a natural regulator of neuron function, and mitochondria are critical to neuron function, including memory formation [344].Local synaptic ATP production must be adjusted to meet high energy demand [345].Mitochondrial mobility in neurons is essential for the formation of memories, and during learning, the number of mitochondria increases, but the size of mitochondria decreases.These mitochondrial changes promote the formation of multicontact synapses, which increases the information storage capacity of new synapses [346].In contrast, in the neurons of the ageing brain, mitochondria become elongated as autophagy, fusion and fission of the mitochondria are disrupted [347].
The abovementioned processes are regulated by ERK1/2, which activate mitochondrial fission and inhibit fusion [185].Cycles of fission and fusion help the mitochondrial network adapt to changing metabolic needs and are part of a fusion-fission-mitophagy quality control pathway enabling the removal of dysfunctional mitochondria.The large GTPase dynamin-related protein 1 (DRP1) is recruited to sites of mitochondrial constriction, where it forms a higher-order ring structure that promotes fission via GTP-dependent scission.The phosphorylation of DRP1 at Ser616 by ERK1/2 promotes mitochondrial translocation of DRP1 and subsequent mitochondrial fission/fragmentation [181,186,187].ERK1/2 have been identified in mitochondria in several independent studies and are strongly regulated by essential mitochondrial products, i.e., CO 2 , ATP and H 2 O 2 [348].Moreover, ERK1/2 regulate mitochondrial biogenesis; in general, they induce mitochondrial biogenesis under control conditions and inhibit it under pathological conditions [188,189].ERK1/2 are involved in the regulation of the transition from mitochondrial respiration to glycolysis [190], and it has been proposed that mitochondrial ERK1/2 provide information about mitochondrial energetic and redox status to the nuclear pathways [349].
Mitochondrial dysfunction is closely associated with a variety of neurological disorders and ultimately leads to neuronal apoptosis.Synaptic mitochondrial dysfunction occurs during ageing and correlates with age-related memory loss.Synaptic mitochondria are the primary targets of both amyloid-β [350,351] and phosphorylated tau [352] toxicity, which contributes to synaptic and memory impairment in Alzheimer's disease.Restoration of mitochondrial function is being intensively developed as a therapeutic strategy for dementia and learning and memory problems [351,353,354].
Understanding the relationship between CO 2 , ERK1/2 and mitochondria may allow for deeper insight into memory mechanisms and the emergence of new therapeutic possibilities.Neurodegenerative disorders are inflammatory in nature and characterized by contradictions in the functioning of ERK1/2; ERK1/2 are essential for the normal function of neurons, but their excessive activity leads to the development of inflammation.Therefore, despite the positive effect of active ERK1/2 on cognition, increased ERK1/2 activity is evident in neurodegenerative disorders.Thus, MAPK inhibition promotes neuroprotection [1,[170][171][172].However, complete inhibition of ERK1/2 induced by synthetic inhibitors can be problematic, since basal ERK1/2 activity is essential for neuronal survival and memory.Elevated CO 2 may be a universal therapeutic alternative that attenuates these drawbacks.CO 2 , on the one hand, may stimulate insufficiently active ERK1/2 (e.g., in an ageing brain) but, on the other hand, inhibit overactive proinflammatory MAPKs.
In addition to the role of mitochondria in the mechanisms underlying memory discussed here, the understanding of the effects of SARS-CoV-2 on mitochondria, including fusion, fission, mitophagy, metabolic reprogramming, and of the regulation of the immune response and apoptosis, has increased, and these effects are thoroughly discussed in numerous recent reviews [355][356][357].

MAPKs and CO 2 in cell survival and apoptosis
Lymphocytes are among the cells whose death contributes most to severe COVID-19, with T-cell apoptosis accounting for T lymphopenia in patients with severe COVID-19 [358].Negative regulation of apoptosis by ERK1/2 has previously been shown to be required to ensure survival of T and B lymphocytes [182].ERK1/2 promote cell survival by activating prosurvival BCL-2 proteins (BCL-2, BCL-xL and MCL1) and repressing prodeath protein (BAD, BIM, BMF and PUMA) activity, including the key mechanism underlying the phosphorylation of BIM EL by ERK1/2, thereby preventing homooligomerization of BAX, a proapoptotic member of the BCL-2 family responsible for the permeabilization of the mitochondrial outer membrane; loss of potential across the inner mitochondrial membrane and cytochrome c release [181,183].
In response to a large cellular imbalance, e.g., caused by DNA damage or excessive inflammation, ERK1/2 are hyperactivated and may exert a proapoptotic effect [4,183].However, in general, ERK1/2 promote cell survival and proliferation, whereas activation of JNK and p38 may induce apoptosis [359].Since a slight increase in CO 2 concentration (from 5 to 8%) activates ERK1/2 and inhibits p38 and JNK under control conditions [10], the question arises: Does such a slight increase in CO 2 levels support the prosurvival activity of ERK1/2 while inhibiting apoptotic p38 and JNK?Indeed, the overlapping functions of CO 2 and ERK1/2 include the regulation of apoptosis and longevity, and the lifespan of mammals positively correlates with blood PaCO 2 and HCO 3 − [5, 167,173,174].Moreover, human cells are unable to proliferate without CO 2 , and elevated CO 2 levels support cell proliferation [175,176]; CO 2 has been shown to exert an effect via ERK1/2 in a cell line derived from human small cell lung cancer [14].Furthermore, hypercapnia induces the expression of anti-apoptotic BCL-2 and BCL-xL and inhibits autophagy in macrophages [178] and ischaemic penumbra astrocytes and neurons [167].Similarly, CAs [179] and acidification [360], which shifts the CO 2 / HCO 3 − equilibrium towards an increase in the level of CO 2 , activate ERK1/2 and thus delay neutrophil apoptosis, improving neutrophil migration and wound healing.Therefore, the inhibition of CAIX resulted in a reduction in the level of active ERK1/2 and reduced neutrophil viability and mitochondrial function [180].An extreme case of the prosurvival effect of CO 2 involves the accelerated growth of the aquatic uniflagellate phycomycetes Blastocladia ramosa and Blastocladia pringsheimii induced by an increase in CO 2 of 5-20% [177].
It is believed that at the time when life was formed, the Earth's atmosphere and water reservoirs contained much more CO 2 than they contain today.To survive, primitive organisms needed to be adapted to the natural environment, i.e., to high concentrations of CO 2 .The change to the oxidizing atmosphere was followed by endosymbiosis, allowing eukaryotes to maintain high levels of CO 2 by producing CO 2 inside the cells in mitochondria.
Typical mitochondrial respiration involving oxygen consumption is associated with CO 2 production during the entry of pyruvate into the Krebs cycle and two stages of the Krebs cycle.Anaerobic energy production by organisms or cells consuming organic compounds is also associated with increased CO 2 levels.However, this production can be mediated by acidification, e.g., by the glycolytic production of lactate, which shifts the CO 2 / HCO 3 − equilibrium towards an increased CO 2 concentration.There are species of multicellular eukaryotes that tolerate periods of complete oxygen deprivation; e.g., the larvae of oriental fruit flies (Bactrocera dorsalis) can tolerate up to 24 h of anoxia without a significant reduction in survival [361].In several species of fish adapted to life under completely oxygen-deprived conditions; for example, one of the clearest differences in crucian carp compared to aerobic species is the activity of pyruvate decarboxylase, which produces CO 2 independent of the Krebs cycle [362,363].In addition, some turtles survive complete anoxic conditions, and upregulation of the prosurvival proteins ERK1/2 and suppression of p38 and JNK underlie neuronal survival [364,365].

Conclusions and future perspectives
Considering the previously published data presented here, it can be concluded that MAPKs play a central role in regulating cellular responses to changing CO 2 levels (Fig. 6).Detailed studies on the regulatory mechanisms MAPK activity by CO 2 suggest that reassessing the functioning of MAPK signalling pathways while taking into account the level of CO 2 , as each MAPK pathway may function differently under altered CO 2 levels, is needed.The effect of CO 2 on the activity of ERK1/2, JNKs and p38 MAPKs varies depending on its concentration.In addition, individual MAPKs function differently in different signalling pathways.Therefore, it can be expected that relatively narrow ranges of CO 2 concentrations will modify MAPK activity in the desired way under specific conditions.Therefore, the basis for future research and the first priority is to determine the effects of specific CO 2 concentrations on individual MAPK isoforms in detail; this research should include in vitro studies using recombinant MAPKs, to confirm the direct CO 2 -sensing ability of MAPKs.We expect that these studies will accelerate CO 2 research because MAPKs are involved in many developmental processes and oncogenesis.
Advances in our understanding of CO 2 signalling have been relatively slow.The first genome-wide proteomic, transcriptomic [366] and genetic [367] analyses of the yeast CO 2 response were reported only recently.These data support the hypotheses presented in this paper by showing that the MAPK pathway is critical for CO 2 sensing and CO 2 signalling in yeast.Hope for progress in CO 2 research is offered via the recent development of selective fluorescent CO 2 molecular sensors, which are expected to lead to breakthrough insights into biochemical processes [368], and detection methods for carboxylation of the amine group in lysine residues [369].
MAPKs are signalling molecules connecting various aspects of the inflammatory response to viral infection, including SARS-CoV-2 infection, and comorbidity pathogenesis.In addition, CO 2 appears to be a molecule that universally counteracts the MAPK-induced proinflammatory response, including in patients with severe course COVID-19 and complications leading to death.The benefits of using elevated CO 2 levels in the treatment of various diseases are reflected in numerous completed and ongoing clinical trials established to evaluated CO 2 use as a medication.Advances in research on the regulation of CO 2 -MAPK may significantly increase the number of new therapeutic applications of CO 2 because a number of MAPK inhibitors have been approved as potent drugs for the treatment of numerous diseases, including cancers, and a significant number of these inhibitors are under evaluation in different stages of clinical trials.However, many MAPK inhibitors cannot be fully exploited at the most effective concentrations due to their toxicity and resistance mechanisms; for example, the activation of ERK5 can enable cells to bypass RAF-MEK1/2-ERK1/2 inhibitors [370].CO 2 , which is safe under controlled conditions [331,371], may overcome these limitations because it inhibits various ERKs, JNKs and p38 MAPKs when administered in a precise concentration range.Other advantages involve the delivery of CO 2 to cells independent of membrane transporters and the possibility of administering very high concentrations of CO 2 In conclusion, understanding the molecular mechanisms underlying CO 2 -dependent regulation of MAPKs, including the opposing effects of elevated CO 2 on active and inactive ERK1/2, is essential to precisely guide the development of therapeutic CO 2 applications.

Fig. 1
Fig. 1 Profiles of MAPK activity in response to elevated CO 2 based on the data shown in Table 1. a CO 2 -dependent regulation of baseline ERK1/2 and JNK levels.The blue dashed line represents the extrapolated JNK activity values.b Inactivation of active MAPKs by CO 2 at elevated levels

Fig. 4 Fig. 5
Fig.4 The physiological and pathological processes regulated by MAPKs and/or CO 2 .Blue lines: mechanisms regulated by MAPKs; red lines: influence of elevated CO 2 levels; black lines: overlapping effects of MAPKs and CO 2

Fig. 6
Fig. 6 Cellular MAPK signalling pathways regulated by CO 2 .Dashed lines represent secondary or concurrent signalling pathways.Multiple arrows indicate indirect regulation

Table 1
Signalling pathways with MAPK activity regulated by CO 2

Table 2
Signalling pathways with overlapping effects of MAPK activity and elevated CO