Fig. 6

STAT3 modulates the methyltransferase function of METTL3 via WTAP. A, B Co-IP analysis of the interaction between METTL3 and WTAP in STAT3 knockdown and control MHCC97H cells (A), STAT3 knockdown and control HCCLM3 cells (B). The lower panels show the quantification of intensity relative to input. C, D The m6A level of total RNA in MHCC97H cells (C) and HCCLM3 cells (D) were determined by the m6A RNA methylation assay kit. The cells were transfected with the indicated siRNAs and/or plasmids. All experiments were repeated at least three times. Error bars represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by 2-tailed Student’s t-test