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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: METTL3 and STAT3 form a positive feedback loop to promote cell metastasis in hepatocellular carcinoma

Fig. 5

STAT3 upregulates WTAP expression by stimulating its transcription. A The diagram shows the five regions which contain the binding sites of STAT3 in the WTAP promoter. B qRT-PCR analysis of the enrichments of five regions in anti-STAT3, Histone3, and IgG immunoprecipitated DNA in MHCC97H (left panel) and HCCLM3 (right panel) cells. C Activities of corresponding fragments of WTAP promoter were examined by luciferase reporter gene assay in HEK293T cells. The cells were transiently transfected with control siRNA or si-STAT3#1 along with indicated fragments of WTAP promoter. D The diagram of pGL3-basic luciferase reporter constructs containing the fragment of human WTAP promoter (pGL-P4). The constructs of wild-type (WT), Delete-3 (the binding site3 (-602 ~ -595) was deleted), Delete-4 (the binding site4 (-454 ~ -447) was deleted), and Delete-5 (the binding site5 (-438 ~ -430) was deleted) were separately shown in the diagram. E Luciferase reporter gene assay of WTAP promoter activities in HEK293T cells. The cells were transiently transfected with control siRNA or si-STAT3#1 and the WTAP promoter (WT) or constructs with deleted binding sites of STAT3. All experiments were repeated at least three times. Error bars represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by 2-tailed Student’s t-test

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