Skip to main content
Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Tricyclic antidepressants induce liver inflammation by targeting NLRP3 inflammasome activation

Fig. 2

Nortriptyline specifically activates NLRP3 inflammasome, rather than AIM2. a-c Cells were incubated with LPS and then pretreated with MCC950 or ODN followed by nortriptyline treatment. Western blot assessed the expression of caspase-1 and IL-1β (a) in SN. The generation of IL-1β (b) or TNF-α (c) in SN was detected via the ELISA kit. (d–f) Nlrp3.−/− BMDMs were incubated with LPS and nortriptyline. The expression of IL-1β and caspase-1 in SN (d) were evaluated by western blot. The levels of IL-1β (e) or TNF-α (f) in SN were measured using ELISA kit. Data are presented as the mean ± SEM (n = 3); ***P < 0.001; ns, not significant; unpaired Student’s t-test (two groups) or One-Way ANOVA followed by the Dunnett's post hoc test (multi groups)

Back to article page