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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles

Fig. 1

Expression level of proteins in the mTOR/4EBP1 and mTOR/P70S6K signalling pathways. (a) Protein expression of p-4EBP1(Thr 37/46), 4EBP1, p-S6K (Thr 389), P70S6K, eIF4E, p-mTOR (Ser 2448), mTOR, p100PI3K; p- indicates phosphorylation. Uncropped western blots for this figure are shown in Additional file 3. (b) Densitometric analysis. The ratio of γ 4EBP1 phosphorylation isoform (hyperphosphorylated form) to levels of 4EBP1 protein. (c) Densitometric analysis. The levels of α-β-γ 4EBP1 phosphorylation isoforms. (d) Densitometric analysis. The ratio of S6K protein phosphorylation on Thr 389 to levels of p70S6K protein. (e) Pearson correlation. The level of p-p70S6K was in negative correlation with the presence of α-4EBP1 isoform (hypophosphorylated) (p ≤ 0.0001) and in positive correlation with γ-4EBP1 isoform (hyperphosphorylated) (p ≤ 0.0001)

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