Fig. 3

A role for PD-L1 in cell migration. A, B Quantification of scratch assay in A BEAS-2B PD-L1 KO cells, N = 6, and B BEAS-2B treated with Durvalumab, N = 4. For A, B rate constants are compared using extra sum-of-squares F test. Cells were in serum-free media for the duration of the assay. C Quantification of scratch assay in H1650 PD-L1 KO/rescue cells, N = 4. Cells were in serum complete media for the duration of the assay. D Quantification of scratch assay in H1650 cells treated with Durvalumab, N = 3. Cells were in serum-free media for the duration of the assay. For C, D difference in slope values is calculated using F-test. E Transwell migration in H1650 PD-L1 KO/rescue cells. One-way ANOVA and post-hoc Tuckey test. F Transwell migration in A549 PD-L1 KO cells. G–I Transwell migration in (G) H1650, H A549 and I H1975 cells treated with Durvalumab. For F–I paired student’s t-test was used on raw values. J Table summarizing cell lines’ response to PD-L1 knock-out or Durvalumab treatment. All error bars = SEM. * ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001