Fig. 2

PD-L1 proximity mapping. A Schematic of PD-L1-APEX2 labeling assay. B Representative epifluorescence image of PD-L1-APEX2 plasma membrane and total stain in BEAS-2B cells. C PM to total distribution of PD-L1-APEX2 in BEAS-2B cells. D PM to total ratio of endogenous PD-L1 and PD-L1-APEX2 construct in BEAS-2B cells treated with vehicle (DMSO) or Akt inhibitor. One-sample t test. E Schematic of experimental design for PD-L1-APEX2 proximity labeling. F Volcano plot of PD-L1-APEX2 labeled proteins. FC = fold change of (labeled/ − ctrl) spectral intensity. Dashed red lines represent fourfold change on the x-axis, and p-value corresponding to FDR = 0.1 after Benjamini–Hochberg correction. G Biological pathways and compartments identified in PD-L1-APEX2 proximity labeling. David gene ontology terms and identifiers are shown. H Additional Biological processes pathways enriched in PD-L1-APEX2 labeling in BEAS-2B. All error bars = SEM. * ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001