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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Paracrine secretion of IL8 by breast cancer stem cells promotes therapeutic resistance and metastasis of the bulk tumor cells

Fig. 2

BCSC secretome confers metastasis. A, B GSEA analysis of EMT associated gene signature A or basal-like associated gene signature B in MCF-7 cells cultured with BCSC CM. C Cell morphology of and F-Actin distribution in MCF-7 and T47D cells cultured with CM from parental cells or BCSCs. D, E Colony-scattering assays D or transwell migration and invasion assay E of MCF-7 and SUM159 cells cultured with CM from parental cells or BCSCs. Scale bar: 300 μm. F Transwell migration and invasion assay of MCF-7 and SUM159 cells co-cultured with the respective parental cells or BCSCs. Scale bar: 300 μm. G Immunoblot of EMT markers in MCF-7 cells cultured with CM from parental cells or BCSCs. H KEGG analysis of pathways enriched in MDA-MB-231 cells cultured with BCSC CM compared to that with control CM. I, J GSEA analysis of the respective gene set enriched in MDA-MB-231 cells cultured with BCSC CM. K The MDA-MB-231 cells were cultured with CM from parental cells or BCSCs for 48 h and then injected into the tail vein of nude mice. In vivo bioluminescent imaging was performed of the metastatic burden 2 weeks later. L The MDA-MB-231 cells were cultured with CM from parental cells or BCSCs for 48 h and orthotopically injected into the second mammary fat pad of nude mice. The primary tumors were excised 2 weeks later and in vivo bioluminescent imaging was performed of the metastatic burden 4 weeks later. All experiments were repeated at least three times. Results are shown as mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant (unpaired two-tailed Student’s t-test)

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