Fig. 5

MMP19 induces E(nd)MT is associated with ET1. A Western blot was performed for ET1 in lung homogenates that MMP19WT- and MMP19E213A- AAV-infected mice treated with saline control or BLM. B Micrographs of immunohistochemical staining of lung sections for ET1. C Expression relevance between MMP19 and ET1 in IPF lung tissues. D ET1 expression level in lung endothelial cells of mice treated with saline and BLM. E Expression relevance between MMP19 and ET1 in lung endothelial cells of mice treated with saline and BLM. F MMP19 and ET-1 were assessed by immunoprecipitation in HPMECs (M = mouse, R = rabbit). G Immunostaining was performed to localize MMP19 (red) and ET-1 (green) in endothelial cells in IPF and mice lung tissues. H HPMECs were pretreated with BOS (10 µM) or DMSO for 24 h, then transient transfection with full-length MMP19 cDNA, and CD31, VE-Cadherin, N-Cadherin, Vimentin and α-SMA were analyzed by Western blotting. I MMP19-AAV-infected mice were pretreated with BLM, then daily gastric infusion of BOS, and CD31, VE-Cadherin, N-Cadherin and Vimentin were analyzed by Western blotting. J Immunofluorescence analysis for CD31 (green) and α-SMA (red) in endothelial cells of MMP19WT-AAV-infected mice treated with BLM and BOS. K Lung hydroxyproline levels in MMP19WT-AAV-infected mice treated with BLM and BOS. n = 5 in all groups. L Micrographs of immunohistochemical staining of lung sections for EMR1. M Representative micrographs of H&E staining and Masson’s trichrome of lung sections