Fig. 3

Clinical data analysis reveals the correlation between RBCK1 and Hippo signaling in TNBC A Compared with breast tissue in TCGA database, RBCK1 mRNA levels in breast cancer data samples increased.. (https://tcga-data.nci.nih.gov). B Compared with normal breast tissue in TCGA database, RBCK1 mRNA levels in all subtypes of breast cancer are increased. (https://tcga-data.nci.nih.gov). C Kaplan–Meier map of progression free survival demonstrates that RBCK1 is associated with good prognosis in triple negative breast cancer patient. (http://kmplot.com/analysis/). D Kaplan–Meier map of progression free survival demonstrates that YAP is associated with poor prognosis in triple negative breast cancer patients. E Gene ontology analysis of the RNA sequencing data shows that RBCK1 depletion in TNBC cells activates the Hippo signaling pathway. MDA-MB-231 cells were transfected with siRBCK1 or siControl. After 48 h, the total mRNA was extracted for RNA sequencing analysis. The siControl and siRBCK1 groups were analyzed in three times. F Gene set enrichment analysis (GSEA) analysis showed that the gene sets of CORDENONSI YAP CONSERVED SIGNATURE was enriched in the RBCK1-low group. G The volcanic map analysis showed that CTGF and CYR61 genes downstream of YAP were significantly up-regulated in the RBCK1-low group. H Cases of tumor showed that RBCK1 protein is negatively correlated with YAP protein in IHC. I Statistical analysis of RBCK1 correlation between RBCK1 and YAP in 40 TNBC tumor samples (Additional file 2)