Fig. 1

RBCK1 depletion promotes cancer cell progression in TNBC A–B RBCK1 deletion efficiency by two different siRNA oligonucleotides in breast cancer cell. *P < 0.05; **P < 0.01; ***P < 0.001 for RBCK1 mRNA level comparison. C–D RBCK1 depletion has no effect on TNBC cancer cell proliferation. Each group was analyzed three times. *P < 0.05; **P < 0.01; ***P < 0.001 for cell growth comparisons. E–F RBCK1 deletion promotes the migration of cell in TNBC.MDA-MB-231 and BT549 cells were transfected with siControl or siRBCK1. Calculate the number of cells and the number data showed as ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 for cell comparisons. G–H RBCK1 depletion promotes the invasion of cell in TNBC.MDA-MB-231 and BT549 cells were transfected with siControl or siRBCK1. Calculate the number of cells and the number data showed as ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 for cell comparisons. I–J Wound healing experiment of TNBC cells was transfected with siRBCK1 or siControl. Wound closure was quantified at a specified point in time. *P < 0.05; ** P < 0.01; ***P < 0.001 for comparisons. K–L RBCK1 depletion inhibited apoptosis in TNBC cells. MDA-MB-231 and BT549 cells were transfected with siControl or siRBCK1. Each group was analyzed three times. *P < 0.05; **P < 0.01; ***P < 0.001 for comparisons. M–N RBCK1 depletion increased the expression of CD24-CD44 + in TNBC cells. MDA-MB-231 and BT549 cells were transfected with siControl or siRBCK1. Each group was analyzed in three times. *P < 0.05; ** P < 0.01; ***P < 0.001 for comparisons