Fig. 5

BVES overexpression inhibited HCC metastasis in vivo. A Representative bioluminescent images of mice in SMMC-7721-Control and SMMC-7721-BVES groups. B Representative liver images of mice in SMMC-7721-Control and SMMC-7721-BVES groups. C The incidence of intrahepatic metastasis in SMMC-7721-Control and SMMC-7721-BVES groups. D The number of intrahepatic metastatic focuses in SMMC-7721-Control and SMMC-7721-BVES groups. E Representative H&E staining images of liver tissues of mice in SMMC-7721-Control and SMMC-7721-BVES groups [scale bars: 200 μm (upper); 100 μm (lower)]. F Representative IF staining images of intrahepatic peri-tumor cell extrusion. The left side of the SMMC-7721-BVES picture was the adjacent nontumor tissues and capsule of nude mice, and the right side was the transplanted tumor in situ. The upper part of the SMMC-7721-Control picture was the orthotopic tumor of the transplanted tumor, and the lower part was the adjacent nontumor tissues and capsule of nude mice. Arrows indicate partially extruded cells. Blue, nuclei; green, BVES (scale bars: 200 μm). Bars represent mean ± S.D., *P < 0.05