Fig. 8
From: Melanoma stimulates the proteolytic activity of HaCaT keratinocytes
Western Blotting analysis of proteins involved in migration and proteolysis. Cell lysates were used for pERK/ERK (A), pAkt/Akt (B), and RUNX2 (C) levels estimation. Conditioned media collected from HaCaTs were utilized for Galectin 3 level evaluation (D). Cells were cultured in the presence of melanoma on Transwell inserts (INS) or with melanoma-conditioned media (CM). Control (CTRL) constitutes of cells cultured in kDMEM: mDMEM (1:1 ratio) media analogously to tested cells. The mean of at least three biological repetitions ± SD is shown. Asterisks indicate statistically important differences between control cells and CAKs or between different type of CAKs. The significance level was set at p ≤ 0.05 (*), p ≤ 0.01 (**) and p ≤ 0.001 (***)