Fig. 6

Functional aquaporins and STIM1-labelled loci in relation to SOCE channels. a Areas with low refractive index are indicated by lower phase density. (Top) Low density areas are found immediately under the plasma membrane (arrowhead), in vesicles near the cell edge, in elevated portions of the cell, and in pointed protrusions (arrow). (Bottom) Linear structures with low density are present in the lamellipodium (bracket). b, c Confocal planes from cells after Ca²⁺ readdition and colocalization with antibodies against STIM1 (blue), Orai (green), and TRPC1 (red). (b, Left) Ventral plane with diffuse Orai (green) and numerous areas of STIM-TRPC colocalization in magenta (arrow). (Right) Dorsal plane of the same cells, c ventral plane of another area showing Orai colocalized with TRPC1 (yellow arrowhead) and areas of STIM1-TRPC1 colocalization (magenta loci). Inset: Nucleus of the same cell showing the diffuse distribution of Orai (green) and colocalization of all three proteins (white). Bars = 10 µm