Fig. 7

The suppression of RLR signaling by MST4 is dependent on its kinase activity. A Luciferase activity of lysates of 293 T cells transfected with empty vector or vector expressing Myc-tagged wild-type MST4 (WT) or MST4 with no kinase activity (KR) or constitutively activated kinase activity (TE) along with IFN-β-Luc or ISRE-Luc for 24 h, and then treated with SeV for 8 h. B Quantitative PCR analysis of IFNB1 mRNA in 293 T cells transfected with empty vector or Myc-MST4-WT, Myc-MST4-KR, or Myc-MST4-TE for 24 h, and then treated with SeV for 8 h. C Secretion of IFN-β by 293 T cells transfected with empty vector or Myc-MST4-WT and Myc-MST4-KR or Myc-MST4-TE for 24 h, and then treated with SeV for 12 h. D Secretion of IFN-β by 293 T-sh-MST4 cells transfected with various combinations of plasmids for 24 h, and then treated with SeV for 12 h. E Immunoblot analysis of lysates of 293 T cells transfected with Myc-MST4-WT, Myc-MST4-KR or Myc-MST4-TE, and Flag-MAVS for 24 h. F Immunoblot analysis of lysates of 293 T cells transfected with Myc-MST4-WT, Myc-MST4-KR, or Myc-MST4-TE for 24 h. G Immunoblot analysis of lysates of 293 T cells transfected with various combinations of plasmids for 24 h and then infected with SeV for 8 h. H Immunoblot analysis of lysates of 293 T cells transfected with Myc-MST4-WT, Myc-MST4-KR or Myc-MST4-TE, Flag-MAVS, and HA-Ub for 24 h, followed by immunoprecipitation with anti-Flag beads. Data information: The data are represented as mean ± SD (A–D: n = 3). *P < 0.05 and **P < 0.01 (unpaired two-tailed Student’s t-test). The data are representative of at least three independent experiments