Fig. 6
From: MST4 negatively regulates type I interferons production via targeting MAVS-mediated pathway
MST4 facilitates K48-linked ubiquitination of MAVS. A Immunoblot analysis of lysates of 293 T cells transfected with Flag-MAVS and increasing amounts of Myc-MST4 for 24 h. B Immunoblot analysis of lysates of 293 T-sh-Control and 293 T-sh-MST4 cells incubated with 100 µg/mL CHX at indicated durations (left). The quantification of the relative expression levels of MAVS (right). C Immunoblot analysis of lysates of 293 T cells transfected with Flag-MAVS, Myc-MST4, or HA-Ub for 24 h, followed by immunoprecipitation with anti-Flag beads. D Immunoblot analysis of lysates of 293 T cells transfected with various combinations of plasmids for 24 h, followed by immunoprecipitation with anti-Flag beads. E Immunoblot analysis of lysates of 293 T-sh-Control and 293 T-sh-MST4 cells infected with SeV for 8 h, followed by immunoprecipitation with control rabbit-IgG or anti-MAVS antibodies. Lysates and immunoprecipitation extracts were probed with K48-Ub, MAVS, and MST4 antibodies. F Immunoblot analysis of lysates of 293 T cells transfected with Flag-AIP4, Flag-Smurf1, or Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. G Immunoblot analysis of lysates of 293 T cells transfected with Flag-Smurf1 or Myc-MST4 and HA-K48-Ub for 24 h, followed by immunoprecipitation with anti-MAVS antibody. Data information: The data are representative of at least three independent experiments