Fig. 5
From: MST4 negatively regulates type I interferons production via targeting MAVS-mediated pathway
MST4 inhibits the binding of TRAF3 to MAVS. A Immunoblot analysis of lysates of 293 T cells transfected with Flag-TRAF3, Myc-MAVS, and increasing amounts of Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. B Immunoblot analysis of lysates of 293 T cells transfected with Flag-IKKε, Myc-MAVS, and increasing amounts of Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. C Immunoblot analysis of lysates of 293 T-sh-Control and 293 T-sh-MST4 cells infected with SeV for 8 h, followed by immunoprecipitation with control rabbit-IgG or anti-TRAF3 antibodies. Lysates and immunoprecipitation extracts were probed with MAVS and TRAF3 antibodies. D Immunoblot analysis of lysates of 293 T cells transfected with Flag-TRAF3, HA-MAVS 360–540, and increasing amounts of Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. E Immunoblot analysis of lysates of 293 T cells transfected with Flag-MAVS, Myc-TRAF3, or Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. Data information: The data are representative of at least three independent experiments